Cloning and Characterization of the Zeaxanthin Glucosyltransferase Gene (crtX) from the Astaxanthin-Producing Marine Bacterium, Paracoccus haeundaensis
Cloning and Characterization of the Zeaxanthin Glucosyltransferase Gene (crtX) from the Astaxanthin-Producing Marine Bacterium, Paracoccus haeundaensis
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DOI：
10.4014/jmb.0904.04033
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Kim, Young Tae
中科院分区：
文献类型：
--
作者：
Seo, Yong Bae;Choi, Seong Seok;Kim, Young Tae
玉米黄质葡萄糖基转移酶（CrtX）介导玉米黄质形成玉米黄质二葡萄糖苷。在这里，我们报告的crtX基因负责玉米黄质二葡萄糖苷的生物合成副球菌haeundaensis和生产相应的类胡萝卜素在转化细胞携带该基因的克隆。构建含有crtX基因的表达质粒（pSTCRT-X），含有该质粒的大肠杆菌细胞产生约46 kDa的重组蛋白。将质粒pSTCRT-X共转化E. pET-44 a（+）-CrtEBIYZ，其携带玉米黄质β-D-二葡萄糖苷生物合成所需的crtE、crtB、crtI、crtY和crtZ基因。
Zeaxanthin glucosyltransferase (CrtX) mediates the formation of zeaxanthin to zeaxanthin diglucoside. Here, we report cloning of the crtX gene responsible for zeaxanthin diglucoside biosynthesis from Paracoccus haeundaensis and the production of the corresponding carotenoids in transformed cells carrying this gene. An expression plasmid containing the crtX gene (pSTCRT-X) was constructed, and Escherichia coli cells containing this plasmid produced the recombinant protein of approximately 46 kDa. Biosynthesis of zeaxanthin diglucoside was obtained when the plasmid pSTCRT-X was co-transformed into E. coli containing the pET-44a(+)-CrtEBIYZ carrying crtE, crtB, crtI, crtY, and crtZ genes required for zeaxanthin beta-D-diglucoside biosynthesis.