Analysis of molecular diversity of bacterial Chitinase genes in the maize rhizosphere using culture-independent methods
Analysis of molecular diversity of bacterial Chitinase genes in the maize rhizosphere using culture-independent methods
复制标题
DOI：
10.1264/jsme2.22.71
复制
发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Fujimura, Tatsuhito
中科院分区：
文献类型：
--
作者：
Ikedai, Seishi;Ytow, Nozomi;Fujimura, Tatsuhito
从玉米根际和非根际土壤的DNA提取物中成功扩增出细菌几丁质酶基因片段。采用末端限制性片段长度多态性和测序分析，在这些土壤样品中的细菌几丁质酶基因的分子多样性进行了评估。我们的研究结果表明，在散装和根际土壤中的细菌几丁质酶的新群体的存在。但根际和非根际土壤中几丁质酶基因的扩增结果存在显著差异，玉米根际优势克隆与阿维链霉菌及其近缘种的几丁质酶基因具有高度的相似性。因此，目前的研究结果表明，培养独立的方法在评估分子多样性之间的几丁质分解细菌群落在土壤中的有用性。
DNA fragments of bacterial chitinase genes were successfully amplified from DNA extracts of maize rhizosphere and bulk soil. The molecular diversity of the bacterial chitinase genes in these soil samples was then evaluated by employing terminal-restriction fragment length polymorphism and sequencing analyses. Our results suggest the presence of novel groups of bacterial chitinase in both bulk and rhizosphere soils. Significant differences were observed, however, between the amplified chitinase genes from rhizosphere and bulk soils, and dominant clones obtained from the maize rhizosphere showed a high degree of similarity to the chitinases of Streptomyces avermitilis and close relatives there of. The current findings thus indicate the usefulness of culture independent methods in the assessment of molecular diversity among the chitinolytic bacterial community in soils.