Protein-bound polysaccharide (PSK) induces cytotoxic activity in the NKL human natural killer cell line
Protein-bound polysaccharide (PSK) induces cytotoxic activity in the NKL human natural killer cell line
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DOI：
10.1007/s005990050079
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发表时间：
1999-12-01
期刊：
影响因子：
--
通讯作者：
Garrido, F
中科院分区：
文献类型：
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作者：
Pedrinaci, S;Algarra, I;Garrido, F
我们研究了蛋白结合多糖PSK对人自然杀伤细胞系NKL活化的影响。当进行标准的2至3小时（51）铬释放试验时，我们观察到针对不同肿瘤细胞（K562、Daudi和U937）的自然杀伤细胞毒活性增加。结果与用白细胞介素-2处理NKL细胞系后获得的结果平行。在100 μ g/ml的PSK浓度下达到最高的细胞毒活性。当PSK剂量为1，000 μ g/ml时，这种自然杀伤细胞活化被抑制。通过荧光激活细胞分选分析的细胞表面标志物在PSK或白细胞介素-2处理NKL细胞后均未显示变化。这些标志物包括CD 2、CD 11b、CD 11 c、CD 18、CD 16、CD 54、CD 56、CD 98、CD 25、CD 122、HLA I类、HLA II类、CD 94、ILT 2、p58.1、p70和NKp 46。这些标志物之一（NKp 46）是一种主要的触发受体，据报道参与新鲜或培养的人自然杀伤细胞的天然细胞毒性。在我们的研究中，另一个触发受体必须牵连在PSK诱导的自然杀伤细胞溶解。我们的数据表明，PSK是一个重要的生物反应调节剂的自然杀伤细胞在体外，并可能被证明是有用的人类自然杀伤细胞生物学的研究。
We studied the effect of protein-bound polysaccharide PSK on the activation of the human natural killer cell line NKL. We observed an increased natural killer cytotoxic activity against different tumor cells (K562, Daudi, and U937) when a standard 2- to 3-h (51)chromium release assay was performed. The results parallel those obtained after treatment of the NKL cell line with interleukin-2. The highest cytotoxic activity was reached at a concentration of 100 mu g/ml of PSK. This natural killer activation was inhibited when the PSK dose was 1,000 mu g/ml. None of the cell surface markers that were analyzed by fluorescence-activated cell sorting showed variations after PSK or interleukin-2 treatment of NKL cells. These markers included CD2, CD11b, CD11c, CD18, CD16, CD54, CD56, CD98, CD25, CD122, HLA class I, HLA class II, CD94, ILT2, p58.1, p70, and NKp46. One of these markers (NKp46) is a major triggering receptor reported to be involved in the natural cytotoxicity of fresh or cultured human natural killer cells. In our study, another triggering receptor must be implicated in PSK-induced natural killer lysis. Our data suggest that PSK is an important biological response modifier of natural killer cells in vitro and may prove to be useful for the study of human natural killer cell biology.