Regulation of adiponectin gene expression in adipose tissue by thyroid hormones
Regulation of adiponectin gene expression in adipose tissue by thyroid hormones
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DOI：
10.1007/s13105-011-0131-1
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发表时间：
2012-06-01
影响因子：
3.4
通讯作者：
Sarkoohi, Parisa
中科院分区：
文献类型：
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作者：
Seifi, Samira;Tabandeh, Mohammad Reza;Sarkoohi, Parisa
现有的实验数据表明，脂联素和甲状腺激素在体内存在生物学相互作用。然而，甲状腺功能不全时甲状腺激素对脂肪脂联素基因表达的影响尚不清楚。我们诱导高-（HYPER）和甲状腺功能减退症（HYPO），每天给药的12毫克/升的左旋甲状腺素和250毫克/升的他巴唑的饮用水的大鼠，分别为42天。在第15、28、42天以及给药停止后2周采集白色脂肪组织和血清样品。采用实时荧光定量PCR和2（-Delta Delta ct）法检测脂联素基因表达。与对照大鼠相比，HYPO大鼠中脂肪组织脂联素mRNA的水平在6周治疗期间降低（< 0.05），并且在HYPO停止后2周显著升高（< 0.05）。脂联素基因表达的这种下降与T3、T4、fT 3和fT 4浓度的降低平行发生（< 0.05）。与HYPO大鼠相反，在6周治疗期间，HYPER大鼠脂肪脂联素基因表达增加，与甲状腺激素浓度增加平行（< 0.05），并且在HYPER停止后2周其表达降低（< 0.05）。脂联素基因表达水平与LDL浓度呈显著负相关（HYPO; =-0.806，= 0.001和HYPER; =-0.749，= 0.002），甘油三酯（HYPO; =-0.825，= 0.001; HYPER; =-0.824，= 0.001），与葡萄糖浓度呈显著正相关（HYPO; = 0.674，= 0.004和HYPER; = 0.866，= 0.001）和HDL（HYPO; = 0.755，= 0.001和HYPER; = 0.839，= 0.001）。目前的研究提供的证据表明，脂肪组织中的脂联素基因表达在翻译水平上受甲状腺激素的调节，甲状腺功能障碍患者的脂质和碳水化合物紊乱可能部分是由于脂联素基因表达的变化。
Available experimental data suggest that adiponectin and thyroid hormones have biological interaction in vivo. However, the effects of thyroid hormones on adipose adiponectin gene expression in thyroid dysfunction are unclear. We induced hyper- (HYPER) and hypothyroidism (HYPO) by daily administration of a 12 mg/l of levothyroxine and 250 mg/l of methimazole in drinking water of rats, respectively, for 42 days. The white adipose tissues and serum sample were taken on days 15, 28, 42 and also 2 weeks after treatment cessation. Analysis of adiponectin gene expression was performed by real-time PCR and 2(-Delta Delta ct) method. The levels of adipose tissue adiponectin mRNA in the HYPO rats were decreased during the 6-week treatment when compared to control rats (< 0.05) and were increased significantly 2 weeks after HYPO cessation ( < 0.05). This decline in adiponectin gene expression occurred in parallel with a decrease in T3, T4, fT3 and fT4 concentrations ( < 0.05). In opposite to HYPO rats, adipose adiponectin gene expression was increased in HYPER rats during the 6-week treatment in parallel with an increase the thyroid hormones concentrations ( < 0.05), and its expression was decreased 2 weeks after HYPER cessation ( < 0.05). Adiponectin gene expression levels showed significant negative correlations with concentrations of LDL (HYPO; = -0.806, = 0.001 and HYPER; = -0.749, = 0.002), triglyceride (HYPO; = -0.825, = 0.001 and HYPER; = -0.824, = 0.001) and significant positive correlations with concentrations of glucose (HYPO; = 0.674, = 0.004 and HYPER; = 0.866, = 0.001) and HDL (HYPO; = 0.755, = 0.001 and HYPER; = 0.839, = 0.001). The current study provides evidence that adiponectin gene expression in adipose tissue is regulated by thyroid hormones at the translation level and that lipid and carbohydrate disturbances in a patient with thyroid dysfunction may be, in part, due to adiponectin gene expression changes.