Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant
Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant
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DOI：
10.1016/j.ydbio.2010.05.013
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Nishida, Hiroki
中科院分区：
文献类型：
--
作者：
Kumano, Gaku;Kawai, Narudo;Nishida, Hiroki
海鞘胚胎的前后轴(A-P轴)是通过后植物皮质/细胞质(PVC)区域的后部化活动来建立的。本文描述了在梭梭胚胎MACO-1中一个新的功能，它是一种母性定位的肌肉决定子，在A-P轴的建立中，除了在肌肉和间质等后部组织的形成和抑制前衍生脊索命运的已知功能外，它作为后部特异性不平等细胞分裂的调节因子，不依赖于它的转录活性。与β-catenin合作，我们的结果表明，macho-1和β-catenin调节微管束的形成，该微管束缩短中心体并将中心体拉向亚细胞皮质结构，称为中心体吸引体(CAB)，该结构位于胚胎的后极，在不等细胞分裂期间，并作用于PEM的上游。最近发现的不平等细胞分裂的调节因子我们还提供的数据表明，PEM定位到CAB可能不是不平等切割调节所必需的。本研究对含锌指转录因子的作用提供了重要的和新的见解，并表明它构成了PVC活性的主要部分(C)2010 Elsevier Inc.保留所有权利
The anterior-posterior (A-P) axis in ascidian embryos is established through the posteriorizing activities of a localized egg region known as the posterior vegetal cortex/cytoplasm (PVC) Here we describe a novel function of macho-1, a maternally-localized muscle determinant, in establishment of the A-P axis in the Halocynthia roretzi embryo Macho-1, in addition to its known function in the formation of posterior tissue such as muscle and mesenchyme, and suppression of the anterior-derived notochord fate, acts Independently of its transcriptional activity as a regulator of posterior-specific unequal cell divisions, In cooperation with beta-catenin Our results suggest that macho-1 and beta-catenin regulate the formation of a microtubule bundle that shortens and pulls the centrosome toward a sub-cellular cortical structure known as centrosome-attracting body (CAB), which is located at the posterior pole of the embryo during unequal cell divisions, and act upstream of PEM, a recently-Identified regulator of unequal cell divisions We also present data that suggest that PEM localization to the CAB may not be required for unequal cleavage regulation The present study provides an important and novel insight into the role of the zinc-finger-containing transcription factor and indicates that it constitutes a major part of the PVC activity (C) 2010 Elsevier Inc All rights reserved