Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by Connexin43
Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by Connexin43
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DOI：
10.3109/15419061.2012.690792
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发表时间：
2012-04-01
影响因子：
--
通讯作者：
Meister, Jean-Jacques
中科院分区：
文献类型：
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作者：
Halidi, Nadia;Alonso, Florian;Meister, Jean-Jacques
血管平滑肌细胞（SMCs）之间的细胞间Ca2+波传播与血管收缩传播有关。在这里，我们描述了间隙连接（GJs）在细胞水平上参与SMCs之间的Ca2+波传播。在A7r5细胞、原代大鼠肠系膜SMCs （pSMCs）和6B5N细胞（A7r5细胞的克隆表达较高的连接蛋白43 (Cx43) / Cx40比值）中，通过细胞间Ca2+波的传播和路西法黄的转移来评估间隙连接通讯。机械刺激诱导pSMC和6B5N细胞内Ca2+波向邻近细胞传播，而A7r5细胞内Ca2+波不能向邻近细胞传播。我们证明了Cx43形成了参与介导细胞间Ca2+波的功能性GJ，并且Cx40与Cx43的共表达，取决于它们的表达比例，可能会干扰Cx43 GJ的形成，从而改变连接通讯。
Intercellular Ca2+ wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca2+ wave propagation between SMCs at the cellular level. Gap junctional communication was assessed by the propagation of intercellular Ca2+ waves and the transfer of Lucifer Yellow in A7r5 cells, primary rat mesenteric SMCs (pSMCs), and 6B5N cells, a clone of A7r5 cells expressing higher connexin43 (Cx43) to Cx40 ratio. Mechanical stimulation induced an intracellular Ca2+ wave in pSMC and 6B5N cells that propagated to neighboring cells, whereas Ca2+ waves in A7r5 cells failed to progress to neighboring cells. We demonstrate that Cx43 forms the functional GJs that are involved in mediating intercellular Ca2+ waves and that co-expression of Cx40 with Cx43, depending on their expression ratio, may interfere with Cx43 GJ formation, thus altering junctional communication.