Inhibitory effects of catalpol on DNCB-induced atopic dermatitis and IgE-mediated mast cells reaction
Inhibitory effects of catalpol on DNCB-induced atopic dermatitis and IgE-mediated mast cells reaction
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DOI：
10.1016/j.intimp.2023.111274
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发表时间：
2023-12-04
影响因子：
5.6
通讯作者：
Tong，Xiaoyun
中科院分区：
文献类型：
--
作者：
Sun，Yun;Zhu，Defen;Tong，Xiaoyun
特应性皮炎(AD)是一种由免疫失调引起的慢性炎症性皮肤病。梓醇是一种环烯醚类化合物，具有抗炎、抗氧化和神经保护作用。它可以作为膳食补充剂添加到食物中。为探讨梓醇对阿尔茨海默病的治疗作用及其机制，我们进行了体内和体外实验。研究发现，梓醇下调Lyn和Syk的磷酸化，抑制多种下游途径，包括细胞内钙升高、细胞因子产生和组胺释放，最终控制肥大细胞(MCs)脱颗粒。结果表明，梓醇可通过调节Th2原和Th2细胞因子，减轻AD样皮损和MC的浸润。此外，该化合物还能降低AD小鼠的IgE水平，并改善PCA小鼠的过敏反应。结果表明，梓醇有可能被开发为一种膳食补充剂，以改善AD和其他特应性疾病。
Atopic dermatitis (AD) is a chronic, inflammatory cutaneous disease driven by immune dysregulation. Catalpol is an iridoids, possessing anti-inflammatory, antioxidant, and neuroprotective activities. It can be added to food as a dietary supplement. To evaluate the effects and mechanisms of catalpol on AD, bothin vitroandin vivostudies were conducted. It was found that catalpol downregulated the phosphorylation of Lyn and Syk to inhibit various downstream pathways, including intracellular Ca2+elevation, cytokines generation, and histamine release, which ultimately controlled mast cell (MCs) degranulation. The results showed that catalpol alleviated AD-like skin lesions and MC infiltration via regulation of pro-Th2 and Th2 cytokinesin vivo. Furthermore, this compound reduced the levels of IgE in AD mice and improved allergic reactions in PCA mice. The results provided that catalpol was potentially developed as a dietary supplement to improve AD and other atopic diseases.