γ-tubulin is essential for microtubule organization and development in Arabidopsis
γ-tubulin is essential for microtubule organization and development in Arabidopsis
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DOI：
10.1105/tpc.105.039644
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发表时间：
2006-06-01
期刊：
影响因子：
11.6
通讯作者：
Bouchez, David
中科院分区：
文献类型：
--
作者：
Pastuglia, Martine;Azimzadeh, Juliette;Bouchez, David
植物细胞微管成核过程一直是植物细胞生物学研究的热点问题。γ -微管蛋白是动物和真菌细胞微管成核的关键分子之一。在这里，我们提供的遗传证据表明，在拟南芥中，伽马微管蛋白是纺锤体、片质体和皮层微管阵列形成所必需的。我们使用反向遗传学方法研究了两个拟南芥γ -微管蛋白基因在植物发育和微管阵列形成中的作用。每个基因的突变体分离和两组γ -微管蛋白双突变体的分析表明，这两个基因具有冗余功能。第一种组合在配子体阶段是致命的。两种γ -微管蛋白基因的破坏会导致纺锤体和片质体结构的异常，并改变配子体的核分裂。γ -微管蛋白等位基因的第二种组合影响幼苗后期发育，最终导致萌发后3周死亡。这种部分存活的突变体组合使我们能够使用绿色荧光蛋白标记动态跟踪γ -微管蛋白耗竭对分裂细胞微管阵列的影响。这些结果确立了-微管蛋白在拟南芥微管阵列形成和组织中的核心作用。
The process of microtubule nucleation in plant cells is still a major question in plant cell biology. gamma-Tubulin is known as one of the key molecular players for microtubule nucleation in animal and fungal cells. Here, we provide genetic evidence that in Arabidopsis thaliana, gamma-tubulin is required for the formation of spindle, phragmoplast, and cortical microtubule arrays. We used a reverse genetics approach to investigate the role of the two Arabidopsis gamma-tubulin genes in plant development and in the formation of microtubule arrays. Isolation of mutants in each gene and analysis of two combinations of gamma-tubulin double mutants showed that the two genes have redundant functions. The first combination is lethal at the gametophytic stage. Disruption of both gamma-tubulin genes causes aberrant spindle and phragmoplast structures and alters nuclear division in gametophytes. The second combination of gamma-tubulin alleles affects late seedling development, ultimately leading to lethality 3 weeks after germination. This partially viable mutant combination enabled us to follow dynamically the effects of gamma-tubulin depletion on microtubule arrays in dividing cells using a green fluorescent protein marker. These results establish the central role of gamma-tubulin in the formation and organization of microtubule arrays in Arabidopsis.