Surface-plasmon-mediated single-molecule fluorescence through a thin metallic film
Surface-plasmon-mediated single-molecule fluorescence through a thin metallic film
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DOI：
10.1103/physrevlett.94.023005
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发表时间：
2005-01-21
影响因子：
8.6
通讯作者：
Kreiter, M
中科院分区：
文献类型：
--
作者：
Stefani, FD;Vasilev, K;Kreiter, M
我们证明，在纳米附近的薄的金膜的单分子的荧光可以有效地激发和检测通过薄膜与落射照明扫描共聚焦显微镜。荧光信号的完整理论处理表明，激发和发射都是表面等离子体介导的。值得注意的是，从垂直于界面的发色团可检测到的光子数因金属的存在而增强。
We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal microscope. A full theoretical treatment of the fluorescence signal indicates that both excitation and emission are surface-plasmon mediated. Remarkably, the number of photons detectable from chromophores perpendicular to the interface is enhanced by the presence of the metal.