Biomimetic porous scaffolds containing decellularized small intestinal submucosa and Sr2+/Fe3+ co-doped hydroxyapatite accelerate angiogenesis/osteogenesis for bone regeneration
Biomimetic porous scaffolds containing decellularized small intestinal submucosa and Sr2+/Fe3+ co-doped hydroxyapatite accelerate angiogenesis/osteogenesis for bone regeneration
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含有脱细胞小肠粘膜下层和 Sr2/Fe3 共掺杂羟基磷灰石的仿生多孔支架可加速骨再生的血管生成/成骨
DOI：
10.1088/1748-605x/ac4b45
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发表时间：
2022-03-01
影响因子：
4
通讯作者：
Guo, Xiaodong
中科院分区：
文献类型：
--
作者：
Cui, Wei;Yang, Liang;Guo, Xiaodong
骨支架的设计主要旨在通过模仿宿主骨的结构/组成来很好地再现天然骨环境。这类仿生生物材料在骨组织工程中具有广阔的应用前景。本文首次通过复杂的自组装矿化过程、交联和冻干后处理制备了含有脱细胞小肠粘膜下层基质（SIS-ECM）和Sr 2 +/Fe 3+共掺杂羟基磷灰石（SrFeHA）的新型仿生骨支架。结果表明，所构建的SIS/SrFeHA支架具有高度多孔结构、粗糙的微表面和较好的机械强度，并能有效释放生物活性Sr 2 +/Fe 3+和ECM成分。这些有利的物理化学性质赋予SIS/SrFeHA支架结构/成分仿生骨环境，这似乎是非常有益的诱导血管生成/成骨在体外和体内。特别是，SIS/SrFeHA支架显著增强了内皮细胞/成骨细胞的细胞功能和生物活性，并且颅骨缺损模型进一步验证了SIS/SrFeHA在植入后加速体内血管化和骨再生的有效能力。从这个角度来看，这些结果突出了仿生多孔SIS/SrFeHA支架用于诱导骨再生的相当大的血管生成/成骨潜力，从而可能为骨组织工程提供一种新的有前途的替代方案。
The design of bone scaffolds is predominately aimed to well reproduce the natural bony environment by imitating the architecture/composition of host bone. Such biomimetic biomaterials are gaining increasing attention and acknowledged quite promising for bone tissue engineering. Herein, novel biomimetic bone scaffolds containing decellularized small intestinal submucosa matrix (SIS-ECM) and Sr2+/Fe3+ co-doped hydroxyapatite (SrFeHA) are fabricated for the first time by the sophisticated self-assembled mineralization procedure, followed by cross-linking and lyophilization post-treatments. The results indicate the constructed SIS/SrFeHA scaffolds are characterized by highly porous structures, rough microsurface and improved mechanical strength, as well as efficient releasing of bioactive Sr2+/Fe3+ and ECM components. These favorable physico-chemical properties endow SIS/SrFeHA scaffolds with an architectural/componential biomimetic bony environment which appears to be highly beneficial for inducing angiogenesis/osteogenesis both in vitro and in vivo. In particular, the cellular functionality and bioactivity of endotheliocytes/osteoblasts are significantly enhanced by SIS/SrFeHA scaffolds, and the cranial defects model further verifies the potent ability of SIS/SrFeHA to accelerate in vivo vascularization and bone regeneration following implantation. In this view these results highlight the considerable angiogenesis/osteogenesis potential of biomimetic porous SIS/SrFeHA scaffolds for inducing bone regeneration and thus may afford a new promising alternative for bone tissue engineering.