Role of the purine repressor hinge sequence in repressor function.
Role of the purine repressor hinge sequence in repressor function.
复制标题
嘌呤阻遏物铰链序列在阻遏物功能中的作用。
DOI：
10.1128/jb.176.6.1767-1772.1994
复制
发表时间：
1994
影响因子：
3.2
通讯作者：
Zalkin,H
中科院分区：
文献类型：
--
作者：
Choi,KY;Zalkin,H
在大肠杆菌嘌呤抑制因子(PURR)中，一个蛋白酶敏感的铰链序列将N-端DNA结合域与相邻的辅阻遏子结合域连接起来。一个二聚体阻遏物分子与操纵者DNA的结合可以保护铰链免受蛋白水解性切割。在活体中，铰链区的突变会削弱抑制物的功能。几个非功能铰链突变体在没有辅阻遏子的情况下与操纵子DNA的低亲和力结合以及与操纵子DNA的高亲和力辅阻遏子依赖的结合都存在缺陷，尽管辅阻遏子的结合类似于野生型抑制子的结合。这些结果确立了铰链区在操纵子结合中的作用，并导致了两条路线形成HoloPurR-操作者复合体的建议。
A protease-hypersensitive hinge sequence in Escherichia coli purine repressor (PurR) connects an N-terminal DNA-binding domain with a contiguous corepressor-binding domain. Binding of one molecule of dimeric repressor to operator DNA protects the hinge against proteolytic cleavage. Mutations in the hinge region impair repressor function in vivo. Several nonfunctional hinge mutants were defective in low-affinity binding to operator DNA in the absence of corepressor as well as in high-affinity corepressor-dependent binding to operator DNA, although binding of corepressor was similar to binding of the wild-type repressor. These results establish a role for the hinge region in operator binding and lead to a proposal for two routes to form the holoPurR-operator complex.