STABILITY OF MATERNAL MESSENGER-RNA IN XENOPUS EMBRYOS - ROLE OF TRANSCRIPTION AND TRANSLATION
STABILITY OF MATERNAL MESSENGER-RNA IN XENOPUS EMBRYOS - ROLE OF TRANSCRIPTION AND TRANSLATION
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DOI：
10.1128/mcb.10.8.4123
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发表时间：
1990-08-01
影响因子：
5.3
通讯作者：
OSBORNE, HB
中科院分区：
文献类型：
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作者：
DUVAL, C;BOUVET, P;OSBORNE, HB
非洲爪哇胚胎的前12个细胞分裂不需要基因转录。这意味着在这一时期基因表达的调控被控制在转录后水平，并使非洲爪哇早期发育成为一个潜在的有趣的生物学系统，用来研究相关的机制。我们在这里描述了几个母体非洲爪哇mRNAs的稳定性特征，它们在受精后不久就被去烯化了(J.Paris和M.Philippe，Dev.传记。)。我们发现，这些mRNAs不仅在胚胎中胚泡转变(MBT)后降解，当时基因转录启动。在MBT后胚胎中，死烯基化的母体mRNAs减少的动力学表现出序列特异性。这些mRNAs在MBT后的降解被放线菌亚胺抑制，但不受放线菌素的影响。因此，这些mRNAs的去稳定化似乎不是由新的胚胎基因转录本启动的。对这些基因3‘’非翻译区的序列比较发现，有几个基序可能与这些基因产物的转录后调控有关。
The first 12 cell divisions of Xenopus laevis embryos do not require gene transcription. This means that the regulation of gene expression during the period is controlled at post-transcriptional levels and makes Xenopus early development a potentially interesting biological system with which to study the mechanisms involved. We described here the stability characteristics of several maternal Xenopus mRNAs which are deadenylated soon after fertilisation (J. Paris and M. Philippe, Dev. Biol., in press). We show that these mRNAs were not only degraded in the embryo after the midblastula transition (MBT), when gene transcription was initiated. The kinetics with which the deadenylated maternal mRNAs decreased in the post-MBT embryos showed sequence specificity. The degradation of these mRNAs after the MBT was inhibited by cycloheximide but was not affected by dactinomycin. Therefore, the destablizatin of these mRNAs does not appear to be initiated by new embryonic gene transcript. Sequence comparisons of the 3'' untranslated region of these mRNA identified several motifs which may be involved in the posttranscritional control of these gene products.