Localization of self-interacting domains within betaretrovirus Gag polyproteins.
Localization of self-interacting domains within betaretrovirus Gag polyproteins.
复制标题
β逆转录病毒 Gag 多蛋白内自相互作用结构域的定位。
DOI：
10.1016/j.virol.2004.12.007
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发表时间：
2005
期刊：
影响因子：
3.7
通讯作者：
Pichová,Iva
中科院分区：
文献类型：
--
作者：
Zábranský,Ales;Sakalian,Michael;Pichová,Iva
The Betaretrovirus genus is characterized by the ability to preassemble immature capsids within the cytoplasm. For Mason–Pfizer monkey virus (M-PMV) this ability depends in part upon the unique Internal Scaffold Domain (ISD) within the p12 region of Gag. In this study, we have further characterized the ability of M-PMV p12 to promote Gag–Gag interaction and have examined the Gag polyprotein of the related mouse mammary tumor virus (MMTV) to potentially identify a region with equivalent function. Using the yeast two-hybrid system, we confirmed that both Gag polyproteins strongly interact, primarily through the CA-NC regions, but also through additional domains N-terminal to CA. For M-PMV, this auxiliary interaction domain was p12. For MMTV, no single strongly self-interacting protein was identified. Instead, MMTV Gag appears to utilize the weak contributions of several protein domains to support the main interaction of its CA-NC. Our findings suggest that, in addition to the canonical NC “I-domain” interaction, MMTV Gag self-association results from the concerted action of multiple regions of the polyprotein while M-PMV Gag relies mainly on its p12 domain.
