RESOLUTION OF THE FLUORESCENCE LIFETIMES IN A HETEROGENEOUS SYSTEM BY PHASE AND MODULATION MEASUREMENTS
RESOLUTION OF THE FLUORESCENCE LIFETIMES IN A HETEROGENEOUS SYSTEM BY PHASE AND MODULATION MEASUREMENTS
复制标题
DOI：
10.1021/j150608a006
复制
发表时间：
1981-01-01
影响因子：
--
通讯作者：
WEBER, G
中科院分区：
文献类型：
--
作者：
WEBER, G
本文描述了一个封闭形式的程序，用于确定衰减常数和相对contributing强度的N个独立的组件的异质荧光发射采用测量的相移和相对调制的总荧光在N个适当的谐波激发频率。在每个频率下，相位和调制测量产生荧光脉冲响应的傅里叶变换的真实的部分G及其虚部S。证明了寿命分布的矩是零矩和偶矩的线性组合或奇矩的线性组合，并导出了在这些线性组合中G和S的系数的构造规则.采用经典的de Prony方法，从矩中得到各组分的寿命和分数贡献。对于二元和三元混合物，所需的数值计算是微不足道的。在现有技术中，二元混合物的组分的寿命应该是可导出的，相对于总体测量的寿命，精度损失略小于1个数量级。
A closed-form procedure is described for the determination of the decay constants and the relativecontributing intensities of the N independent components of a heterogeneous fluorescence emission employing measurements of the phase shift and relative modulation of the total fluorescence at N appropriateharmonic excitation frequencies. At each frequency the phase and modulation measurements yield the real part of the Fourier transform of the fluorescence impulse response, G, and its imaginary part, S. It is shown that the moments of a distribution of the lifetimes are linear combinations of the Gs (zero and even moments) or the Ss (odd moments), and the rule for the construction of the coefficients of G and S in these linear combinations is derived. The classical de Prony method is used to obtain the lifetimes and fractional contributionsof the components from the moments. For binary and ternary mixtures the numerical computations required are trivial. In the present state of the art, the lifetimes of the components of a binary mixture should be derivable with a loss in precisionsomewhat smaller than 1 order of magnitude with respect to the overall measured lifetimes.