Immunohistochemical characterisation of pelvic autonomic ganglia in male mice
Immunohistochemical characterisation of pelvic autonomic ganglia in male mice
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DOI：
10.1007/s00441-002-0673-1
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发表时间：
2003-02-01
影响因子：
3.6
通讯作者：
Keast, JR
中科院分区：
文献类型：
--
作者：
Wanigasekara, Y;Kepper, ME;Keast, JR
盆神经节是交感-副交感神经混合神经节，为泌尿生殖器官提供大部分自主神经支配。在这里，我们描述的结构和组织化学特征的主要盆神经节在雄性小鼠和比较两个不同的小鼠品系。神经节的基本结构特征与雄性大鼠相似。几乎所有的盆神经节细胞都是单极的，大多数是胆碱能的。所有这些都含有神经肽Y（NPY）或血管活性肠肽（VIP），或两种肽一起。肽共存在品系之间变化，C57 BL/6小鼠具有与单独的NPY、单独的VIP或两种肽相似的神经元比例。相反，几乎所有的盆神经元Quacken-bush-Swiss（QS）株表达NPY，即VIP/NPY共存的水平要高得多。胆碱能轴突为生殖器官、膀胱平滑肌和阴茎勃起组织的上皮细胞提供主要的神经供应。它们还提供前列腺、精囊和输精管的平滑肌神经支配的次要成分。事实上，所有非胆碱能的盆神经节细胞都是去甲肾上腺素能的，并且含有NPY。它们的主要靶点是生殖器官的平滑肌。这项研究表明，雄性小鼠盆腔神经节与大鼠有许多相似之处，但VIP/NPY共定位在小鼠中更为常见。我们还表明，有不同品系的小鼠副交感神经盆腔神经元肽的表达。这些研究为今后研究泌尿生殖功能的神经调节提供了框架，特别是在转基因和基因敲除模型中。
Pelvic ganglia are mixed sympathetic-parasympathetic ganglia and provide the majority of the autonomic innervation to the urogenital organs. Here we describe the structural and histochemical features of the major pelvic ganglion in the male mouse and compare two different mouse strains. The basic structural features of the ganglion are similar to those in the male rat. Almost all pelvic ganglion cells are monopolar and most are cholinergic. All contain either neuropeptide Y (NPY) or vasoactive intestinal peptide (VIP), or both peptides together. The peptide coexistence varies between strains, with C57BL/6 mice having similar proportions of neurons with NPY alone, VIP alone or both peptides. In contrast, virtually all pelvic neurons in the Quacken-bush-Swiss (QS) strain express NPY, i.e. the level of VIP/NPY coexistence is much higher. Cholinergic axons provide the major nerve supply to epithelia of reproductive organs, bladder smooth muscle and, as described previously, penile erectile tissue. They also provide a minor component of the smooth muscle innervation of the prostate gland, seminal vesicles and vas deferens. Virtually all non-cholinergic pelvic ganglion cells are noradrenergic and contain NPY. Their major target is smooth muscle of reproductive organs. This study shows that the male mouse pelvic ganglion bears many similarities to that in the rat, but that VIP/NPY colocalisation is much more common in the mouse. We also show that there are differences in peptide expression in parasympathetic pelvic neurons between strains of mice. These studies provide the framework for future investigations on neural regulation of urogenital function, particularly in transgenic and knockout models.