Single glass nanopore-based regenerable sensing platforms with a non-immobilized polyglutamic acid probe for selective detection of cupric ions
Single glass nanopore-based regenerable sensing platforms with a non-immobilized polyglutamic acid probe for selective detection of cupric ions
复制标题
基于单玻璃纳米孔的可再生传感平台，具有非固定化聚谷氨酸探针，用于选择性检测铜离子
DOI：
10.1016/j.aca.2015.06.051
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发表时间：
2015-08-19
影响因子：
6.2
通讯作者：
Jin, Yongdong
中科院分区：
文献类型：
--
作者：
Chen, Lizhen;He, Haili;Jin, Yongdong
利用聚谷氨酸(PGA)作为非固定化探针，建立了一种基于单玻璃毛细管纳米孔的铜离子快速、选择性检测的传感平台。这种检测是基于在铜离子存在下，通过纳米孔的离子电流显著减少，以及在探针上的络合铜离子引起的离子电流整流反应的逆转。与其他金属离子相比，PGA对铜离子的检测具有较高的选择性。通过在测量过程中采用非对称的盐梯度，传感平台的灵敏度可以提高约1-2个数量级。基于PGA的纳米孔传感平台在Cu2+传感应用中表现出良好的可再生性。此外，该方法对于小体积样品中铜离子的检测是有效和可靠的，最小体积为20毫升。这种基于纳米孔的传感平台将在铜离子检测中找到很有前途的实际应用。(C)2015爱思唯尔B.V.保留所有权利。
A single glass capillary nanopore-based sensing platform for rapid and selective detection of cupric ions is demonstrated by utilizing polyglutamic acid (PGA) as a non-immobilized probe. The detection is based on the significant decrease of ionic current through nanopore and the reversal of ion current rectification responses induced by the chelated cupric ions on the probes when in the presence of cupric ions. PGA shows high selectivity for detecting cupric ions rather than other metal ions. The sensitivity of the sensing platform can be improved about 1-2 orders of magnitude by employing asymmetric salt gradients during the measurements. And the PGA-based nanopore sensing platform shows excellent regenerability for Cu2+ sensing applications. In addition, the method is found effective and reliable for the detection of cupric ions in real samples with small volume down to 20 mL. This nanopore-based sensing platform will find promising practical applications for the detection of cupric ions. (C) 2015 Elsevier B.V. All rights reserved.