Development of mammary preneoplasias in vivo from mouse mammary epithelial cell lines in vitro.
Development of mammary preneoplasias in vivo from mouse mammary epithelial cell lines in vitro.
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DOI：
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发表时间：
1992-04
期刊：
影响因子：
11.2
通讯作者：
F. Kittrell;C. J. Oborn;D. Medina
中科院分区：
文献类型：
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作者：
F. Kittrell;C. J. Oborn;D. Medina
一系列的小鼠乳腺上皮细胞系已建立了一个协议，得到高度可重复的结果。根据角蛋白中间丝的阳性免疫染色、波形蛋白中间丝的阴性免疫染色、酪蛋白的激素诱导以及在体内表现出导管和腺泡乳腺形态发生的能力，判定乳腺上皮细胞系（指定为FSK系）为上皮细胞。FSK细胞系在低血清（1%）培养基中依赖于表皮生长因子和胰岛素。梭形细胞培养物的条件培养基取代了对血清的需求，并使FSK 3和FSK 4在胶原凝胶中的生长分别增加了4-5倍，在单层培养中增加了12-14倍。在第2-11代注射到乳腺脂肪垫中后，FSK细胞系产生稳定的可移植增生性肺泡生长系。根据其在体内稳定的肺泡形态和对肿瘤发生的易感性增加，将体内生长系判断为癌前病变。FSK细胞系及其衍生物体内生长系提供了一种新的和潜在的生产系统，以检查乳腺癌前病变的发展中涉及的关键分子改变。此外，体外培养系统的再现性提供了小鼠乳腺上皮细胞的稳定细胞系可以容易地和随意地产生的保证。
A series of mouse mammary epithelial cell lines has been established by a protocol that gives highly reproducible results. The mammary epithelial cell lines, designated as FSK lines, were judged to be epithelial based on positive immunostaining for keratin-intermediate filaments, negative immunostaining for vimentin-intermediate filaments, hormonal induction of casein, and the ability to exhibit ductal and alveolar mammary morphogenesis in vivo. The FSK cell lines are dependent on epidermal growth factor and insulin in a low serum (1%) medium. Conditioned medium from spindle cell cultures replaced the requirement for serum and increased the growth of FSK3 and FSK4 4-5 times in collagen gels and 12-14 times in monolayer culture, respectively. Following injection into the mammary fat pad at passages 2-11, the FSK cell lines generated stable transplantable hyperplastic alveolar outgrowth lines. The in vivo outgrowth lines were judged as preneoplastic based on their stable alveolar morphology in vivo and an increased susceptibility for tumorigenesis. The FSK cell lines and their derivative in vivo outgrowth lines provide a new and potentially productive system to examine critical molecular alterations involved in the development of mammary preneoplasias. Furthermore, the reproducibility of the in vitro culture system provides the assurance that stable cell lines of mouse mammary epithelial cells can be generated easily and at will.