Accelerated evolution of crotalinae snake venom gland serine proteases
Accelerated evolution of crotalinae snake venom gland serine proteases
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DOI：
10.1016/s0014-5793(96)01144-1
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发表时间：
1996-11-11
期刊：
影响因子：
3.5
通讯作者：
Ohno, M
中科院分区：
文献类型：
--
作者：
Deshimaru, M;Ogawa, T;Ohno, M
从草鱼毒腺和草鱼毒腺cDNA文库中分离到的8个编码丝氨酸蛋白酶的cDNA表明，在成熟蛋白编码区发生了非同义的核苷酸替换，引起氨基酸的改变。用两个合适的探针对黄绿色葡萄球菌基因组DNA进行Southern杂交分析表明，毒腺丝氨酸蛋白酶基因在基因组中形成一个多基因家族。这些观察表明，毒腺丝氨酸蛋白酶以一种加速的方式使其氨基酸序列多样化。由于先前在龟尾蛇毒腺磷脂酶A(2)(pla(2))同工酶基因中发现了类似的特征，因此加速进化似乎在Crotalinae蛇毒腺的多个同工酶家族中普遍存在。
Eight cDNAs encoding serine proteases isolated from Trimeresuras flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes, Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been pre,iously discovered in crotalinae snake venom gland phospholipase A(2) (PLA(2)) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.