Cytoplasmic-Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines
Cytoplasmic-Nuclear Genomic Barriers in Rice Pollen Development Revealed by Comparison of Global Gene Expression Profiles among Five Independent Cytoplasmic Male Sterile Lines
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DOI：
10.1093/pcp/pcq026
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发表时间：
2010-04-01
影响因子：
4.9
通讯作者：
Toriyama, Kinya
中科院分区：
文献类型：
--
作者：
Fujii, Sota;Yamada, Mari;Toriyama, Kinya
细胞质雄性不育（Cytoplasmic male不育，CMS）是目前已知的高等植物中描述线粒体-核基因组相互作用不亲和的最理想现象之一。为了阐明花粉发育对线粒体基因型和细胞质-核基因组屏障的依赖性，我们采用了5个具有不同花粉缺陷表型的水稻CMS等基因系，分别是CW-、W11-、LD-、BT-和wa型CMS系，并结合它们的线粒体基因组结构对CMS表型和核基因表达模式进行了表征。这5个不育系携带独立的有丝分裂型，W11、LD和BT线粒体基因组在系统发育上相对接近。在单核小孢子和双细胞花粉期的花药中，8199个基因在至少一个CMS系中显著改变了表达。k-means聚类后，BT、LD和W11表达模式相同。在编码线粒体蛋白的基因中，已知的线粒体胁迫标记基因ALTERNATIVE OXIDASE 1A被包括在BT、LD和W11双细胞花粉期花药中异位上调的基因组中。在花粉发育过程中，其他几个簇也以细胞质特异性的方式受到调控。BT-、LD-和W11-CMS系基因调控网络的这些明显相似性表明，线粒体基因型的系统发育关系与核基因表达模式和花粉败育表型密切相关，为花粉发育过程中线粒体对核基因组的上位性提供了证据。
Cytoplasmic male sterility (CMS) is one of the most ideal phenomena known in higher plants to describe the incompatibilities between mitochondrial-nuclear genomic interactions. To elucidate the dependency of pollen development on mitochondrial genotypes and cytoplasmic-nuclear genomic barriers, we employed five CMS isogenic lines of rice, CW-, W11-, LD-, BT- and WA-type CMS lines, that exhibit distinct pollen-defective phenotypes, and we characterized the CMS phenotypes and the nuclear gene expression patterns in conjunction with their mitochondrial genomic structures. These five CMS lines carried independent mitotypes, and W11, LD and BT mitochondrial genomes were relatively close with respect to their phylogeny. In anthers at the uninucleate microspore and bicellular pollen stages, 8,199 genes significantly changed their expression in at least one of the CMS lines. Common expression patterns were observed in BT, LD and W11 after k-means clustering. Among the genes encoding putative mitochondrial proteins, ALTERNATIVE OXIDASE 1A, a gene for the well-known mitochondrial stress marker, was included in the group ectopically up-regulated in anthers at the bicellular pollen stage of BT, LD and W11. Several other clusters were also regulated in a cytoplasm-specific manner during pollen development. These clear similarities in gene regulatory networks of BT-, LD- and W11-CMS lines indicate that the phylogenetic relationships of the mitochondrial genotypes are strongly correlated with nuclear gene expression patterns and pollen abortion phenotypes, providing evidence of the mitochondrial epistacy over the nuclear genome during pollen development.