The mitochondrial DNA copy number, cytochrome c oxidase activity and reactive oxygen species level in metaphase II oocytes obtained from in vitro culture of cryopreserved ovarian tissue in comparison with in vivo-obtained oocyte
The mitochondrial DNA copy number, cytochrome c oxidase activity and reactive oxygen species level in metaphase II oocytes obtained from in vitro culture of cryopreserved ovarian tissue in comparison with in vivo-obtained oocyte
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DOI：
10.1111/jog.13747
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发表时间：
2018-10-01
影响因子：
1.6
通讯作者：
Ghorbanmehr, Nassim
中科院分区：
文献类型：
--
作者：
Amoushahi, Mahboobeh;Salehnia, Mojdeh;Ghorbanmehr, Nassim
目的：为探讨玻璃化冷冻培养的小鼠卵巢组织中线粒体DNA（mtDNA）拷贝数、活性氧（ROS）水平和线粒体酶活性，以非玻璃化冷冻组和体内获得的卵母细胞为对照。然后，分离腔前卵泡并在三维培养系统中进行培养。评价并比较两组卵泡发育和卵母细胞成熟情况。收集一些来自体外和体内条件下的中期II卵母细胞与获能精子授精，然后，受精率和胚胎发育率进行了评估。结果：两组体外培养的中期II期卵母细胞的胚胎发育、线粒体DNA拷贝数和线粒体酶活性均显著低于体内培养组（P < 0. 05），而ROS水平则显著高于体内培养组（P < 0. 05）。
Aim: To evaluate the mitochondrial DNA (mtDNA) copy number, reactive oxygen species (ROS) level and intensity of mitochondrial enzyme activity in metaphase II oocytes derived from vitrified cultured immature mouse ovarian tissue in comparison with nonvitrified group and in vivo-obtained oocytes.Methods: Vitrified and nonvitrified ovaries from neonate female mice were cultured for 7days. Then, preantral follicles were isolated and cultured in a three-dimensional culture system. Follicular development and oocyte maturation were evaluated and compared in both groups. Some of the collected metaphase II oocytes derived from in vitro and in vivo conditions were inseminated with capacitated spermatozoa, and then, the fertilization and embryo developmental rates were assessed. In the other series of oocytes, mtDNA copy number, distribution and enzyme activity and ROS level were analyzed.Results: The embryo development, mtDNA copy number and mitochondrial enzyme activity in collected metaphase II oocytes from two in vitro-cultured groups were significantly lower, and the ROS level was higher than those of the in vivo group (P