Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production
Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production
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DOI：
10.3389/fchbi.2024.1362147
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发表时间：
2024-03
期刊：
影响因子：
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通讯作者：
S. Brooks;J. Weaver;Anthikan Klomchit;Shumukh A. Alharthi;Thanyarat Onlamun;Rithika Nurani;
中科院分区：
文献类型：
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作者：
S. Brooks;J. Weaver;Anthikan Klomchit;Shumukh A. Alharthi;Thanyarat Onlamun;Rithika Nurani;
内生真菌构成了次生代谢物的丰富来源，可以通过操纵这些代谢物来生产所需的新型类似物，以应对当前作物生产的农业挑战，特别是控制植物病害。内生真菌 Daldinia eschscholtzii MFLUCC 19-0629 是从热带古老植物 Oncosperma sp. 中新分离出来的，对多种植物病原体表现出广谱的抗真菌和抗菌活性，包括青枯菌、尖孢镰刀菌、胶孢炭疽菌、锐角炭疽菌、鹿角孢菌cucurbitacearum、Corynespora cassiicola 和 Stemphylium spp.使用牛津纳米孔技术获得了 D. eschscholtzii MFLUCC 19-0629 的高质量基因组序列，读数的准确性和长度导致不需要 Illumina 或其他测序技术，从而产生 37.56 Mb 的基因组大小，由 11 个大小相当的重叠群组装而成，可能处于染色体水平。生物信息学分析表明，该菌株具有生物合成能力，编码 67 个预测的生物合成基因簇 (BGC)。 67 个 BGC 中只有 8 个与先前表征的与已知次生代谢物的产生相关的 BGC 匹配或表现出高度相似性。大量预测的未知 BGC 使该菌株成为新型天然产物的有前途的来源。 D. eschscholtzii MFLUCC 19-0629 对与作物生产相关的七种主要植物病原微生物具有广谱抗菌活性，其完整基因组序列的发现对于新型微生物生物防治剂 (MBCA) 的发展具有极其重要的意义。这也揭示了发现可用于可持续农业和制药目的的新化合物的前景。
Endophytic fungi constitute a rich source of secondary metabolites that can be manipulated to produce desirable novel analogs for combating current agricultural challenges for crop production, especially controlling plant disease. The endophytic fungus Daldinia eschscholtzii MFLUCC 19-0629, was newly isolated from tropical ancient plants, Oncosperma sp., and displays a broad-spectrum of antifungal and antibacterial activities against several plant pathogens including Ralstonia solanacearum, Fusarium oxysporum, Colletotrichum gloeosporioides, Colletotrichum acutatum, Stagonosporopsis cucurbitacearum, Corynespora cassiicola and Stemphylium spp. A high-quality genome sequence was obtained using Oxford nanopore technology, the accuracy and length of reads resulting in no need for Illumina or other sequencing techniques, for D. eschscholtzii MFLUCC 19-0629, resulting in a genome size of 37.56 Mb assembled over 11 contigs of significant size, likely to be at the chromosomal level. Bioinformatics analysis revealed that this strain is biosynthetically talented encoding 67 predicted biosynthetic gene clusters (BGCs). Only eight of the 67 BGCs matched or demonstrated high similarity to previously characterized BGCs linked to the production of known secondary metabolites. The high number of predicted unknown BGCs makes this strain a promising source of novel natural products. The discovery that D. eschscholtzii MFLUCC 19-0629 has a broad spectrum of antimicrobial activity against seven major plant pathogenic microorganisms relevant to crop production and its complete genome sequence carries immense importance in the advancement of novel microbial biocontrol agents (MBCAs). This also unveils the prospect of uncovering new compounds that could be utilized for sustainable agriculture and pharmaceutical purposes.