Vibrational spectroscopic changes of B-lymphocytes upon activation.

Vibrational spectroscopic changes of B-lymphocytes upon activation.
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B 淋巴细胞激活后的振动光谱变化。

DOI:
10.1002/jbio.201200136
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发表时间:
2013
影响因子:
2.8
通讯作者:
Bird,Benjamin
Bird,Benjamin
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Mazur,AntonellaI;Monahan,JenniferL;Miljkovic,Milos;Laver,Nora;Diem,Max;Bird,Benjamin

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报道了使用振动显微光谱成像解释正常淋巴结中B淋巴细胞活化的第一项研究。淋巴细胞活化表明病原体的存在和对病原体的反应,而不管激发病原体的病因,无论是良性的、反应性的还是恶性的过程。了解疾病早期淋巴细胞活化和免疫反应的生化组成,可能有助于确定肿瘤的起源,而不存在恶性转移细胞,但在淋巴结内是反应性的,并显示增生区域。红外和拉曼数据通过无监督的多变量方法可以提供一种物理的和可重复的方法来确定活化淋巴结的生化成分和其中的变化,其特征取决于该区域或身体其他地方存在的恶性肿瘤。本文报告的结果提供了一项概念验证研究,揭示了在不存在转移细胞的情况下筛查淋巴结疾病的潜力。(© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA,魏因海姆)
The first study interpreting B‐lymphocyte activation in normal lymph nodes using vibrational micro‐spectral imaging is reported. Lymphocyte activation indicates the presence and response against a pathogen, regardless of the inciting pathogen's etiology, whether a benign, reactive or malignant process. Understanding the biochemical makeup of lymphocyte activation during early stages of disease and immune response may offer significant aid in determining a tumor's origin without the presence of malignant metastatic cells but within lymph nodes that are reactive and displaying regions of hyperplasia. Infrared and Raman data scrutinizedviaunsupervised multivariate methods may provide a physical and reproducible method to determine the biochemical components and variances therein of activated lymph nodes with distinguishing characteristics depending on the malignancy present in the region or elsewhere in the body. The results reported here provide a proof‐of‐concept study that reveal a potential to screen lymph nodes for disease without the presence of metastatic cells. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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DOI: --
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