BIOCHEMICAL-STUDIES ON CELL-FUSION .1. LIPID-COMPOSITION OF FUSION-RESISTANT CELLS
BIOCHEMICAL-STUDIES ON CELL-FUSION .1. LIPID-COMPOSITION OF FUSION-RESISTANT CELLS
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DOI：
10.1083/jcb.101.4.1578
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发表时间：
1985-01-01
影响因子：
7.8
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
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作者：
ROOS, DS;CHOPPIN, PW
先前已分离到一系列稳定的小鼠成纤维细胞突变体(Roos，D.S.和R.L.Davidson，1980 Body Cell Genet.，6：381-390)，它们对聚乙二醇(PEG)的融合诱导作用表现出不同程度的抗性，但在形态上与其来源的亲本细胞相似。对这些突变细胞系的生化分析表明，它们的全细胞脂组成存在差异，这与它们对融合的敏感性直接相关。抗融合细胞含有水平升高的中性脂质，特别是甘油三酯和一种不寻常的乙醚连接的脂类，O-烷基，二酰甘油。这个乙醚类物质增加了。apprx。在对聚乙二醇抗性最强的细胞系中，亲本细胞是亲本细胞的35倍。抗融合细胞还含有更多高度饱和的脂肪酰链(饱和脂肪酸与多不饱和脂肪酸的比率[S/P比率].apprxeq.4：1)，高于亲本(S/P比.apprxeq.1：1)。对聚乙二醇抗性居中的细胞具有介于亲本细胞和最耐融合突变体之间的乙醚、脂肪和脂肪酸组成。在一个相关的通信(Roos，D.S.和P.W.Choppin，1985，J.Cell.Biol.，100：1591-1598)提出的证据表明，脂类含量的变化可以预测地控制这些细胞的融合反应。
A series of stable cell mutants of mouse fibroblasts were previously isolated (Roos, D.S. and R.L. Davidson, 1980 Somatic Cell Genet., 6:381-390) that exhibit varying degrees of resistance to the fusion-inducing effect of polyethylene glycol (PEG), but are morphologically similar to the parental cells from which they were derived. Biochemical analysis of these mutant cell lines has revealed differences in whole cell lipid composition which are directly correlated with their susceptibility to fusion. Fusion-resistant cells contain elevated levels of neutral lipids, particularly triglycerides and an unusual ether-linked lipid, O-alkyl, diacylglycerol. This ether lipid is increased .apprx. 35-fold over parental cells in the most highly PEG-resistant cell line. Fusion-resistant cells also contain more highly saturated fatty acyl chains (ratio of saturated to polyunsaturated fatty acids [S/P ratio] .apprxeq. 4:1) than the parental line (S/P ratio .apprxeq. 1:1). Cells which are intermediate in their resistance to PEG have ether lipid and fatty acid composition which is intermediate between the parental cells and the most fusion-resistant mutants. In a related communication (Roos, D.S. and P.W. Choppin, 1985, J. Cell. Biol., 100:1591-1598) evidence is presented that alteration of lipid content can predictably control the fusion response of these cells.