Astroglia in medullary dorsal horn (trigeminal spinal subnucleus caudalis) are involved in trigeminal neuropathic pain mechanisms.
Astroglia in medullary dorsal horn (trigeminal spinal subnucleus caudalis) are involved in trigeminal neuropathic pain mechanisms.
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DOI：
10.1523/jneurosci.3365-09.2009
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发表时间：
2009-09-09
期刊：
影响因子：
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通讯作者：
Iwata K
中科院分区：
文献类型：
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作者：
Okada-Ogawa A;Suzuki I;Sessle BJ;Chiang CY;Salter MW;Dostrovsky JO;Tsuboi Y;Kondo M;Kitagawa J;Kobayashi A;Noma N;Imamura Y;Iwata K
本研究的目的是调查是否星形胶质细胞在延髓背角（三叉神经脊神经尾侧亚核; Vc）可能参与口面神经病理性疼痛后三叉神经损伤。鞘内给予星形胶质细胞顺乌头酸酶抑制剂氟乙酸钠（FA）对下肺泡神经横断（IANX）大鼠Vc星形胶质细胞过度活跃（通过胶质细胞酸性蛋白（GFAP）标记显示）、伤害反应行为、Vc细胞外信号调节激酶磷酸化（pERK）和Vc神经元活性的影响。与假手术对照组大鼠相比，IANX大鼠术后第7天同侧Vc中GFAP阳性细胞显着增加，但FA给药后这种情况得到了阻止。FA显着增加了减少头撤回潜伏期高强度热刺激的上颌胡须垫皮肤在IANX大鼠，虽然它没有显着影响降低逃避阈值低强度机械刺激的胡须皮肤在IANX大鼠。FA还显著降低Vc中pERK样免疫反应细胞数量的增加，以及在IANX大鼠中记录的高强度皮肤刺激后增强的Vc伤害性神经元反应，谷氨酰胺给药恢复了增强的反应。这些不同的研究结果提供了第一个文件，星形胶质细胞参与增强的伤害性反应的功能鉴定Vc伤害性神经元和相关的口面痛觉过敏三叉神经损伤后。
The aim of this study was to investigate whether astroglia in the medullary dorsal horn (trigeminal spinal subnucleus caudalis; Vc) may be involved in orofacial neuropathic pain following trigeminal nerve injury. The effects of intrathecal administration of the astroglial aconitase inhibitor sodium fluoroacetate (FA) were tested on Vc astroglial hyperactivity [as revealed by glial fibrillary acid protein (GFAP) labeling], nocifensive behavior, Vc extracellular signal-regulated kinase phosphorylation (pERK), and Vc neuronal activity in inferior alveolar nerve-transected (IANX) rats. Compared with sham-control rats, a significant increase occurred in GFAP-positive cells in ipsilateral Vc at postoperative day 7 in IANX rats, which was prevented following FA administration. FA significantly increased the reduced head withdrawal latency to high-intensity heat stimulation of the maxillary whisker pad skin in IANX rats, although it did not significantly affect the reduced escape threshold to low-intensity mechanical stimulation of the whisker skin in IANX rats. FA also significantly reduced the increased number of pERK-like immunoreactive cells in Vc and the enhanced Vc nociceptive neuronal responses following high-intensity skin stimulation that were documented in IANX rats, and glutamine administration restored the enhanced responses. These various findings provide the first documentation that astroglia is involved in the enhanced nociceptive responses of functionally identified Vc nociceptive neurons and in the associated orofacial hyperalgesia following trigeminal nerve injury.