BACTERIAL MUTANTS IN WHICH GENE N FUNCTION OF BACTERIOPHAGE LAMBDA IS BLOCKED HAVE AN ALTERED RNA POLYMERASE
BACTERIAL MUTANTS IN WHICH GENE N FUNCTION OF BACTERIOPHAGE LAMBDA IS BLOCKED HAVE AN ALTERED RNA POLYMERASE
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DOI：
10.1073/pnas.68.12.2977
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
GEORGOPO.CP
中科院分区：
文献类型：
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作者：
GEORGOPO.CP
细菌突变体已被分离出来，称为groN，它通过干扰噬菌体N基因产物的作用来阻止噬菌体发育。色氨酸酶的合成依赖于色氨酸酶的产物，但色氨酸噬菌体不会使这些酶进入细菌内。已经分离出两种可以克服groN阻断的噬菌体突变体。一种类型产生改变的N产物，另一种包含N旁路突变。groN突变与大肠杆菌中的利福霉素抗性位点密切相关。与来自gro+的聚合酶相比，来自thegroN突变体的纯化RNA聚合酶较少被盐激活并且对利福霉素更敏感。这表明groN突变导致细菌RNA聚合酶发生结构变化，使其无法再与噬菌体产物正确相互作用。
Bacterial mutants have been isolated, calledgroN, that block phage development by interference with the action of the product of the phageNgene. λtrpphages, which depend on theNproduct for the synthesis of tryptophan enzymes, do not make these enzymes ingroNbacteria. Two type of phage mutants have been isolated that can overcome thegroNblock. One type makes an alteredNproduct, the other contains anN-bypass mutation.ThegroNmutation is closely linked to the rifamycin-resistance locus inEscherichia coli. Purified RNA polymerase from thegroNmutant is less activated by salt and more sensitive to rifamycin than is the polymerase fromgro+. This suggests that thegroNmutation produces a structural change in the bacterial RNA polymerase such that it can no longer interact properly with the phageNproduct.