Comparison of three chromatographic techniques for the detection of mitragynine and other indole and oxindole alkaloids in Mitragyna speciosa (kratom) plants.
Comparison of three chromatographic techniques for the detection of mitragynine and other indole and oxindole alkaloids in Mitragyna speciosa (kratom) plants.
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DOI：
10.1002/jssc.201301389
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发表时间：
2014-06
影响因子：
3.1
通讯作者：
Mei Wang;Emily J. Carrell;Z. Ali;B. Avula;C. Avonto;J. Parcher;I. Khan
中科院分区：
文献类型：
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作者：
Mei Wang;Emily J. Carrell;Z. Ali;B. Avula;C. Avonto;J. Parcher;I. Khan
东南亚植物帽柱木的叶子可用于抑制疼痛和缓解阿片类药物戒断综合症。这种不受控制的精神活性植物的潜在滥用威胁和容易获得，导致需要改进分析技术来检测主要活性成分帽柱木碱和 7-羟基帽柱木碱。比较了与两种检测系统联用的三种独立色谱方法，即 GC 与 MS、超临界流体色谱与二极管阵列检测以及 HPLC 与 MS 和二极管阵列检测，用于分析帽柱木碱和其他吲哚和羟吲哚生物碱。吲哚生物碱包括两组非对映异构体：(i) paynantheine 和 3-isopaynantheine 和 (ii) 帽柱木碱、speciogynine 和 spiociliatine。还研究了两种羟吲哚生物碱非对映异构体：Corynoxine 和 Corynoxine B。 HPLC 和超临界流体色谱方法成功分离了主要成分，但洗脱顺序略有不同。 GC 方法不太令人满意，因为它无法分离帽柱木碱和特奇西利亚汀。通过使用液体固定相的 GC 很难进行这种分离，因为这些非对映异构体仅在内部氢原子的方向上有所不同。观察到的吲哚生物碱非对映异构体缺乏分辨率，加上质谱和串联质谱的相似性，对仅基于 GC 和 MS 分离和鉴定的帽柱木碱分析所提出的 GC 方法提出了质疑。
Leaves of the Southeast Asian plant Mitragyna speciosa are used to suppress pain and mitigate opioid withdrawal syndromes. The potential threat of abuse and ready availability of this uncontrolled psychoactive plant have led to the need for improved analytical techniques for the detection of the major active components, mitragynine and 7-hydroxymitragynine. Three independent chromatographic methods coupled to two detection systems, GC with MS, supercritical fluid chromatography with diode array detection, and HPLC with MS and diode array detection, were compared for the analysis of mitragynine and other indole and oxindole alkaloids in M. speciosa plants. The indole alkaloids included two sets of diastereoisomers: (i) paynantheine and 3-isopaynantheine and (ii) mitragynine, speciogynine, and speciociliatine. Two oxindole alkaloid diastereoisomers, corynoxine and corynoxine B, were also studied. The HPLC and supercritical fluid chromatography methods successfully resolved the major components with slightly different elution orders. The GC method was less satisfactory because it was unable to resolve mitragynine and speciociliatine. This separation was difficult by GC with a liquid stationary phase because these diastereoisomers differ only in the orientation of an interior hydrogen atom. The observed lack of resolution of the indole alkaloid diastereoisomers coupled with the likeness of the mass and tandem mass spectra, calls into question proposed GC methods for the analysis of mitragynine based on solely GC with MS separation and identification.