A SINGLE PHOSPHOTYROSINE RESIDUE OF STAT91 REQUIRED FOR GENE ACTIVATION BY INTERFERON-GAMMA
A SINGLE PHOSPHOTYROSINE RESIDUE OF STAT91 REQUIRED FOR GENE ACTIVATION BY INTERFERON-GAMMA
复制标题
DOI：
10.1126/science.7690989
复制
发表时间：
1993-09-24
期刊：
影响因子：
56.9
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
SHUAI, K;STARK, GR;DARNELL, JE
干扰素-γ（IFN-γ）通过诱导91千道尔顿细胞质蛋白（称为STAT，信号转导子和转录激活子）的酪氨酸磷酸化来刺激特定基因的转录。Stat 91在单一位点（Tyr 701）上磷酸化，并且该位点的磷酸化是核转位、DNA结合和基因激活所需的。Stat 84是同一基因的差异剪接产物，缺少Stat 91的38个羧基末端氨基酸，尽管它被磷酸化并易位到细胞核并结合DNA，但它不激活转录。因此，Stat 91介导响应IFN-γ的转录激活。
Interferon-gamma (IFN-gamma) stimulates transcription of specific genes by inducing tyrosine phosphorylation of a 91-kilodalton cytoplasmic protein (termed STAT for signal transducer and activator of transcription). Stat91 was phosphorylated on a single site (Tyr701), and phosphorylation of this site was required for nuclear translocation, DNA binding, and gene activation. Stat84, a differentially spliced product of the same gene that lacks the 38 carboxyl-terminal amino acids of Stat91, did not activate transcription, although it was phosphorylated and translocated to the nucleus and bound DNA. Thus, Stat91 mediates activation of transcription in response to IFN-gamma.