Viral miRNAs in plasma and urine divulge JC polyomavirus infection.
Viral miRNAs in plasma and urine divulge JC polyomavirus infection.
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DOI：
10.1186/1743-422x-11-158
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发表时间：
2014-09-02
期刊：
影响因子：
4.8
通讯作者：
Stuyver LJ
中科院分区：
文献类型：
--
作者：
Lagatie O;Van Loy T;Tritsmans L;Stuyver LJ
JC多瘤病毒（JCPyV）是一种广泛存在的人类多瘤病毒，通常潜伏在宿主体内，但在免疫功能低下的情况下可被重新激活，可能导致进行性多灶性白质脑病（PML）。JCPyV编码自身的microRNA jcv-miR-J1。我们在50名健康受试者中调查了jcv-miR-J1-5p（及其变体jcv-miR-J1a-5p）是否可以在血浆或尿液中检测到。我们发现JCPyV miRNA在血浆中的总检出率为74%(37/50)，在尿液中的总检出率为62%（31/50）。根据JCPyV VP1血清学状态和病毒脱落情况进一步对受试者进行分类。在血清阴性受试者中，86%（12/14）和57%（8/14）的血浆和尿液样本中检测到JCPyV miRNA。血清阳性受试者血浆和尿液检出率分别为69%（25/36）和64%（23/36）。此外，在尿中病毒脱落的血清阳性受试者中，与未脱落的血清阳性受试者相比，观察到更高水平的尿病毒mirna （P < 0.001）。尿液和血浆mirna之间没有相关性。这些数据表明，对循环病毒mirna的分析揭示了潜伏的JCPyV感染的存在，从而进一步对血清阳性个体进行分层。此外，我们的数据表明，感染率比单独的血清学预期的要高。本文的在线版本（doi:10.1186/1743-422X-11-158）包含补充材料，可供授权用户使用。
JC polyomavirus (JCPyV) is a widespread human polyomavirus that usually resides latently in its host, but can be reactivated under immune-compromised conditions potentially causing Progressive Multifocal Leukoencephalopathy (PML). JCPyV encodes its own microRNA, jcv-miR-J1. We have investigated in 50 healthy subjects whether jcv-miR-J1-5p (and its variant jcv-miR-J1a-5p) can be detected in plasma or urine. We found that the overall detection rate of JCPyV miRNA was 74% (37/50) in plasma and 62% (31/50) in urine. Subjects were further categorized based on JCPyV VP1 serology status and viral shedding. In seronegative subjects, JCPyV miRNA was found in 86% (12/14) and 57% (8/14) of plasma and urine samples, respectively. In seropositive subjects, the detection rate was 69% (25/36) and 64% (23/36) for plasma and urine, respectively. Furthermore, in seropositive subjects shedding virus in urine, higher levels of urinary viral miRNAs were observed, compared to non-shedding seropositive subjects (P < 0.001). No correlation was observed between urinary and plasma miRNAs. These data indicate that analysis of circulating viral miRNAs divulge the presence of latent JCPyV infection allowing further stratification of seropositive individuals. Also, our data indicate higher infection rates than would be expected from serology alone. The online version of this article (doi:10.1186/1743-422X-11-158) contains supplementary material, which is available to authorized users.