Respiratory neuron group in the high cervical spinal cord discovered by optical imaging
Respiratory neuron group in the high cervical spinal cord discovered by optical imaging
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DOI：
10.1097/wnr.0b013e328318edb5
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发表时间：
2008-11-19
期刊：
影响因子：
1.7
通讯作者：
Okada, Yasumasa
中科院分区：
文献类型：
--
作者：
Oku, Yoshitaka;Okabe, Akihito;Okada, Yasumasa
脊椎动物的自发呼吸是短暂的，表明颈脊髓产生呼吸节律。我们使用电压敏感染料记录了新生大鼠离体脑干-脊髓制备的光学信号，并利用相关系数成像技术对光学数据进行处理，以可视化呼吸相关活动。我们发现了一个新的区域，从髓-脊髓连接处延伸到C2节，距离腹表面约100-300 μ m深，被称为高颈呼吸群。我们记录了该区域的吸气神经元和吸气前-吸气神经元。高颈呼吸组神经元不同于运动神经元，因为它们体积小，呈纺锤形，只有两到三个长突起，因此可能是参与颈脊髓呼吸节律发生的中间神经元。神经报告19:1739-1743 (C) 2008沃尔特斯克鲁威健康| Lippincott Williams & Wilkins。
Spontaneous breathing occurs transiently in spinalized animals suggesting that the cervical spinal cord generates respiratory rhythm. We recorded optical signals from isolated brainstem-spinal cord preparations of neonatal rats using a voltage-sensitive dye, and visualized respiratory-related activity by processing the optical data with a correlation coefficient imaging technique. We identified a novel region that extended from the medullo-spinal junction to the C2 segment, approximately 100-300 mu m deep from the ventral surface, and termed the high cervical respiratory group. We recorded inspiratory and preinspiratory-inspiratory neurons in this region. High cervical respiratory group neurons are distinct from motoneurons, because they are small, spindle in shape, and have only two or three long processes, and thus may be interneurons that are involved in respiratory rhythmogenesis in the cervical spinal cord. NeuroReport 19:1739-1743 (C) 2008 Wolters Kluwer Health | Lippincott Williams & Wilkins.