Electron capture dissociation of gaseous multiply charged ions by Fourier-transform ion cyclotron resonance
Electron capture dissociation of gaseous multiply charged ions by Fourier-transform ion cyclotron resonance
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DOI：
10.1016/s1044-0305(00)00223-3
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发表时间：
2001-03-01
影响因子：
3.2
通讯作者：
Carpenter, BK
中科院分区：
文献类型：
--
作者：
McLafferty, FW;Horn, DM;Carpenter, BK
傅里叶变换离子回旋共振仪器是唯一适用于一个不寻常的新的离子化学，电子捕获解离（ECD）。这导致比先前观察到的（10 kDa）离子大得多的分子（42 kDa）的非遍历解离，如果它们首先被热激活，则产生更广泛的ECD。这种高特异性的共价键切割也使得ECD有希望研究的二级和三级结构的气态蛋白质离子所造成的非共价键。(C)2001年美国质谱学会。
Fourier-transform ion cyclotron resonance instrumentation is uniquely applicable to an unusual new ion chemistry, electron capture dissociation (ECD). This causes nonergodic dissociation of far larger molecules (42 kDa) than previously observed ( 10 kDa) ions give far more extensive ECD if they are first thermally activated. This high specificity for covalent bond cleavage also makes ECD promising for studying the secondary and tertiary structure of gaseous protein ions caused by noncovalent bonding. (C) 2001 American Society for Mass Spectrometry.