Temperature-jump studies of merocyanine 540 relaxation kinetics in lipid bilayer membranes.
Temperature-jump studies of merocyanine 540 relaxation kinetics in lipid bilayer membranes.
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脂双层膜中部花青 540 弛豫动力学的温跃研究。
DOI：
10.1021/bi00346a015
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Frosch,MP
中科院分区：
文献类型：
--
作者：
Verkman,AS;Frosch,MP
材料和方法化学品。M-540获自Molecular Probes，Inc.（JunctionCity，OR）。卵磷脂（PC）购自Makor Chemicals Ltd。（耶路撒冷，以色列），并在硅胶G（ICN Nutritional Biochemicals，Cleveland，OH）上使用氯仿-甲醇-水（65：25：4）进行薄层色谱后得到单一斑点。所有其他化学品均为试剂级，并从Sigma Chemical Co. (St.密苏里州路易斯）。
Materials and MethodsChemicals. M-540 was obtained from Molecular Probes, Inc.(JunctionCity, OR). Egg phosphatidylcholine (PC) was obtained fromMakor Chemicals Ltd.(Jerusalem, Israel) and gave a single spot after thin-layer chromatography on silica gel G (ICN Nutritional Biochemicals, Cleveland, OH) using chloroform-methanol-water (65: 25: 4). All otherchemicals were of reagent grade and were obtained from Sigma Chemical Co.(St. Louis, MO).