Repair of human sperm chromosome aberrations in the hamster egg
Repair of human sperm chromosome aberrations in the hamster egg
复制标题
仓鼠卵子中人类精子染色体畸变的修复
DOI：
10.1007/bf00217120
复制
发表时间：
1992
期刊：
影响因子：
5.3
通讯作者：
J. Egozcue
中科院分区：
文献类型：
--
作者：
A. Genescà;M. Caballín;R. Miró;J. Benet;J. Germá;J. Egozcue
为了研究受精卵对人类精子存在或诱导的损伤的修复能力，我们检测了DNA修复抑制剂咖啡因对精子染色体畸变率和类型的增强作用。精子样本是由三年前因睾丸癌接受化疗的个人捐赠的。精子和受精卵母细胞暴露于咖啡因后，精子染色体畸变率增加，表明人类精子的损伤可以在未经处理的地鼠卵细胞质中修复。咖啡因的增强作用主要体现在增加未连接的畸变率，表明染色体重排的形成也受到抑制。由于咖啡因处理后染色单体类型和染色体类型的异常都增加了，人类精子的损伤可能可以通过复制前和复制后的修复机制在仓鼠卵细胞质内修复。
SummaryIn order to study the repair capacity of fertilized hamster eggs for the lesions present or induced in human sperm, we have examined the potentiating effect of caffeine, a DNA repair inhibitor, on the frequency and types of sperm chromosome aberrations. Sperm samples were donated by an individual treated with chemotherapy for a testicular cancer 3 years previously. Exposure of spermatozoa and inseminated oocytes to caffeine led to an increase of sperm chromosome aberrations, indicating that the damage to human sperm can be repaired in untreated hamster egg cytoplasm. The potentiating effect of caffeine was mainly reflected in an increase of unrejoined aberrations, indicating that the formation of chromosomal rearrangements is also inhibited. Since both chromatid-type and chromosome-type aberrations increase after treatment with caffeine, damage to human sperm can probably be repaired inside the hamster egg cytoplasm by pre and post-replication repair mechanisms.
咖啡因和 3-氨基苯甲酰胺作为叙利亚幼仓鼠肾细胞 DNA 修复抑制剂的比较分析。
DOI：
10.1016/0167-8817(84)90013-0
发表时间：
1984
期刊：
Mutation research
影响因子：
--
作者：
Das,SK;Lau,CC;Pardee,AB
通讯作者：
Pardee,AB