DETERMINATION OF SEVEN BIFLAVONES OF SELAGINELLA DOEDERLEINII BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DETERMINATION OF SEVEN BIFLAVONES OF SELAGINELLA DOEDERLEINII BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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高效液相色谱法测定卷柏中7种双黄酮的含量
DOI：
10.1080/00032719.2013.831426
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发表时间：
2013-12-12
影响因子：
2
通讯作者：
Lin, Xinhua
中科院分区：
文献类型：
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作者：
Li, Shaoguang;Yao, Hong;Lin, Xinhua
卷柏(Selaginella doederleinii Hieroi，S.doederleinii)在亚洲被广泛用作抗肿瘤和抗炎药物，并以含有双黄酮类化合物而闻名，双黄酮类化合物已被报道为具有抗癌和抗氧化作用的活性成分。然而，目前还缺乏一种简便、系统的双黄酮类成分的质量控制方法。在本研究中，从杜氏链霉菌中分离得到7个双黄酮类化合物，包括双黄酮类、罗布斯塔夫酮、2，3-二氢-3，3-二氢-3，3-联苯二异黄酮、3，3-双黄酮素、去甲黄酮类、七叶黄酮类和7，4，7，4-四-O-甲基-双黄酮类。以这7种高浓度化合物为检测标记物，采用高效液相色谱-紫外可见检测器建立了质量控制方法。色谱柱为C-18柱，流动相为乙腈-水(0.5%冰醋酸)，时间为52min。在优化条件下，7种双黄酮类成分在实验范围内呈良好的回归关系(R-2和GT；0.9989)，回收率在90.1-102.5%之间。7种分析物的检出限在0.2~1.17微克/毫升之间。该方法的精密度和准确度也得到了验证，并成功地用于不同产地的6批杜氏链霉菌中这些化合物的含量测定。综上所述，本研究为毛冬青药材的质量评价提供了一种系统、实用的方法。
Selaginella doederleinii Hieron (S. doederleinii) is widely used as an antitumor and anti-inflammatory agent in Asia, and is famous for containing biflavonoids, which have been reported as the bio-active constituents possessing anticancer and anti-oxidant effects. However, a simple and systematic quality control method for determining biflavonoids in S. doederleinii is lacking. In this study, seven biflavones, including amentoflavone, robustaflavone, 2, 3-dihydro-3, 3-biapigenin; 3, 3-binaringenin, delicaflavone, heveaflavone, and 7, 4, 7, 4-tetra-O-methyl-amentoflavone, were obtained from S. doederleinii. These seven compounds that are present in high concentration were used as detection markers for development of a quality control method by HPLC with a UV-visible detector. The chromatographic separation was accomplished on a C-18 column with a mobile phase composed of acetonitrile-water (0.5% acetic acid) in 52min. With the optimized conditions, the seven biflavones showed good regression relationships (R-2>0.9989) within the test range and the recovery was in the range of 90.1-102.5%. The LODs of the seven analytes were between 0.2 and 1.17 mu g/mL. The method was also validated for precision and accuracy and was successfully applied to the determination of these compounds in six batches of S. doederleinii from different areas. In conclusion, the present study provides a systematic and useful method for quality evaluation of S. doederleinii.