Characterization of a SRS13: a new cyst wall mucin-like domain containing protein.
Characterization of a SRS13: a new cyst wall mucin-like domain containing protein.
复制标题
SRS13 的表征：一种新的囊壁粘蛋白样结构域蛋白。
DOI：
10.1007/s00436-018-5934-3
复制
发表时间：
2018
影响因子：
2
通讯作者：
Weiss,Louis
中科院分区：
文献类型：
--
作者：
Tomita,Tadakimi;Ma,Yanfen;Weiss,Louis
刚地弓形虫是一种专性细胞内顶复体，约三分之一的人类感染弓形虫。在潜伏感染期，T.在包囊内发现了生殖迟缓子，包囊是一种改良的寄生虫空泡。这种寄生虫具有SRS（表面抗原-1相关序列）蛋白的大家族，据报道，SRS蛋白参与这些生物体与其哺乳动物宿主细胞的附着以及潜伏感染期间的免疫破坏。我们已经确定了一种新的粘蛋白结构域包含SRS蛋白，使用糖表位特异性抗体，它识别的囊肿壁。SRS 13具有两个SRS结构域，并且在这些结构域之间是富含苏氨酸的串联重复粘蛋白样结构域，其类似于在另一种囊壁特异性SRS蛋白CST 1（SRS 44）中看到的粘蛋白样结构域。SRS 13在缓殖子和被ppGalNAc-T2和T3糖基化的O-GalNAc中上调。与囊壁蛋白CST 1类似，SRS 13定位于囊壁，但与CST 1不同，SRS 13是正常囊壁形成的抑制剂。总之，这些发现阐明了第二SRS粘蛋白结构域蛋白，SRS 13，在缓殖子生物学中的作用，并扩展了以前报道的SRS蛋白家族的功能。
Toxoplasma gondii, an obligate intracellular apicomplexan, causes latent infection in about one third of the human population. During latent infection,T. gondiibradyzoites are found within cysts, a modified parasitophorous vacuole. This parasite has a large family of SRS (surface antigen-1 related sequence) proteins which are reported to be involved in attachment of these organisms to their mammalian host cells and in immune subversion during latent infection. We have identified a novel mucin domain containing SRS protein, using a glycoepitope-specific antibody, which recognizes the cyst wall. SRS13 has two SRS domains and between these domains is a threonine-rich tandem repeat mucin-like domain that is similar to the mucin-like domain seen in another cyst wall specific SRS protein CST1 (SRS44). SRS13 is upregulated in bradyzoites andO-GalNAc glycosylated by ppGalNAc-T2 and T3. Similar to the cyst wall protein CST1, SRS13 localizes to the cyst wall, but unlike CST1, SRS13 is dispensable for normal cyst wall formation. Together, these findings elucidate the role of a second SRS mucin domain protein, SRS13, in bradyzoite biology and expands the previously reported functions of the SRS protein family.