Histological and lectin histochemical studies of the vomeronasal organ of horses
Histological and lectin histochemical studies of the vomeronasal organ of horses
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DOI：
10.1016/j.tice.2016.05.002
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发表时间：
2016-08-01
期刊：
影响因子：
2.6
通讯作者：
Shin, Taekyun
中科院分区：
文献类型：
--
作者：
Lee, Kwang-Hyup;Park, Changnam;Shin, Taekyun
用组织学、免疫组织化学和凝集素组织化学方法研究了马犁鼻器(VNO)的形态特征和糖结合物组成。VNO位于双侧鼻中隔底部，具有被软骨包围的管状结构，由感觉上皮和非感觉上皮组成。免疫组织化学染色显示，犁鼻感觉上皮(VSE)由嗅觉标记蛋白(OMP)和蛋白基因产物9.5(PGP 9.5)阳性的受体细胞、支持细胞和基底细胞组成。VNO受体细胞呈G蛋白Gα(I2)阳性，但不表达Gα(O)。用21种生物素标记的凝集素进行的凝集素组织化学研究表明，VSE的游离缘与20种凝集素呈阳性反应。受体和支持细胞与16种凝集素反应，基底细胞与15种不同强度的凝集素反应。在犁鼻无感觉上皮中，19种凝集素的游离缘呈阳性。纤毛细胞17种凝集素阳性，基底细胞15种凝集素阳性。位于固有层的犁鼻腺被高碘酸希夫(PAS)和阿利新蓝(pH 2.5)染色。18种凝集素以不同的结合模式对犁鼻腺腺泡细胞进行染色。这些结果表明，马的VNO受体细胞表达1型犁鼻受体，并且VNO腺体具有粘液到浆液的特征。此外，每种凝集素都不同地结合了VNO感觉和非感觉上皮中的每种细胞类型。(C)2016爱思唯尔有限公司。保留所有权利。
The morphological characteristics and glycoconjugate composition of the vomeronasal organ (VNO) of the horse was investigated using histological, immunohistochemical, and lectin histochemical methods. The VNO is bilaterally located at the base of the nasal septum, has a tubular structure surrounded by cartilage, and consists of sensory and non-sensory epithelia. Immunohistochemical examination showed that the vomeronasal sensory epithelium (VSE) consisted of receptor cells positive for both olfactory marker protein (OMP) and protein gene product 9.5 (PGP 9.5), supporting cells, and basal cells. VNO receptor cells were positive for G protein G alpha(i2) (vomeronasal receptor type 1 marker), but not G alpha(o) (vomeronasal receptor type 2 marker). Lectin histochemical studies using 21 biotinylated lectins showed that the free border of the VSE was positive for 20 lectins. The receptor and supporting cells reacted with 16 lectins while the basal cells reacted with 15 lectins, with varying intensities. In the vomeronasal non-sensory epithelium, the free border was positive for 19 lectins. The cilated cells were positive for 17 lectins and the basal cells were positive for 15 lectins. The vomeronasal glands, positioned in the lamina propria, were stained with both periodic acid Schiff (PAS) and alcian blue (pH 2.5). Eighteen lectins stained the acinar cells of the vomeronasal glands with various binding patterns. These findings suggest that horse VNO receptor cells express vomeronasal receptor type 1, and the VNO glands have mucous to seromucous characteristics. Moreover, each lectin differentially binds each cell type in both the VNO sensory and non-sensory epithelia. (C) 2016 Elsevier Ltd. All rights reserved.