CHARACTERIZATION OF ANTERIOR-PITUITARY TARGET-CELLS FOR ARGININE VASOPRESSIN - INCLUDING CELLS THAT STORE ADRENOCORTICOTROPIN, THYROTROPIN-BETA, AND BOTH HORMONES
CHARACTERIZATION OF ANTERIOR-PITUITARY TARGET-CELLS FOR ARGININE VASOPRESSIN - INCLUDING CELLS THAT STORE ADRENOCORTICOTROPIN, THYROTROPIN-BETA, AND BOTH HORMONES
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DOI：
10.1210/endo-125-1-554
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发表时间：
1989-07-01
期刊：
影响因子：
4.8
通讯作者：
UNABIA, G
中科院分区：
文献类型：
--
作者：
CHILDS, GV;WESTLUND, KN;UNABIA, G
精氨酸加压素（AVP）刺激CTH和TSH的分泌。最近，亲和细胞化学技术与一个有效的生物素类似的AVP被用来确定ACTH细胞作为靶细胞之一。计数显示AVP结合的细胞占群体的10%。然而，如果AVP结合所有的促肾上腺皮质激素和促甲状腺激素，那么人们预期AVP结合至少16%的垂体细胞。因此，使用双重细胞化学标记方案来了解促甲状腺激素是否也结合AVP（生物AVP）。48%的AVP靶细胞含有ACTH，42%含有TSH β。AVP结合细胞的百分比增加到12-13%的总垂体细胞后1小时的预处理在10 nM TRH或CRH。TRH和CRH共同刺激增加到总细胞的16%。双标记的分析表明，额外的AVP结合细胞刺激CRH或TRH源于促肾上腺皮质激素或促甲状腺激素的人口，分别。促甲状腺激素释放激素刺激TSH细胞的百分比增加，结合AVP从55%到75%。类似地，CRH刺激结合AVP的ACTH细胞的百分比从61%增加到79%。此外，标记TSH β的细胞群也可以被标记。或ACTH抗原在未标记的AVP中1小时后增加30%，支持其对这些靶细胞的直接作用。促甲状腺激素释放激素刺激TSH细胞类似的增量。CRH预处理对TSH或ACTH标记的细胞百分比没有影响。这可能是鉴定刺激促肾上腺皮质激素所需的ACTH储备丢失的结果。最后，分析AVP结合的TSH β的总百分比。或促肾上腺皮质激素细胞表明在人口重叠。这刺激了aCTH和TSH β的双重标记的应用。在仅暴露于溶剂的人群中，1-2%的混合垂体细胞储存ACTH和TSH。这种独特的细胞类型还包括10%的通过逆流离心富集的促皮质激素群体。AVP作用1h后，ACTH-TSH细胞占混合细胞的比例增加到4.8%。它不受TRH或CRH（或两种肽）的影响。这些研究表明AVP靶细胞包括促甲状腺细胞、促皮质细胞和储存ACTH和TSH的独特细胞。
Arginine vasopressin (AVP) stimulates the secretion of CTH and TSH. Recently, affinity cytochemical techniques with a potent biotinylated analog of AVP were used to identify ACTH cells as one of the target cells. Counts showed that AVP-bound cells were 10% of the population. However, if AVP bound all corticotropes and thyrotropes, one would expect AVP to bind at least 16% of the pituitary cells. Therefore, dual cytochemical labeling protocols were used to learn if thyrotropes also bound AVP (bio-AVP). Forty-eight percent of AVP target cells contained ACTH, and 42% contained TSH.beta.. The percentages of AVP-bound cells were increased to 12-13% of the total pituitary cells after 1-h pretreatment in 10 nM TRH or CRH. TRH and CRH together stimulated increases to 16% of the total cells. Analysis of dual labels showed that the additional AVP-bound cells stimulated by CRH or TRH stemmed from the corticotrope or thyrotrope populations, respectively. TRH stimulated an increase in the percentage of TSH cells that bound AVP from 55% to 75%. Similarly, CRH stimulated an increase in the percentage of ACTH cells that bound AVP from 61% to 79%. In addition, the populations of cells labeled for TSH.beta. or ACTH antigens increased by 30% after 1 h in unlabeled AVP, supporting its direct effect on these target cells. TRH stimulated a similar increment in TSH cells. CRH pretreatment had no effect on the percentages of cells labeled for TSH or ACTH. This could be the result of loss of ACTH stores needed to identify stimulated corticotropes. Finally, analysis of the total percentages of AVP-bound TSH.beta. or ACTH cells suggested an overlap in the population. This stimulated the application of dual labels for aCTH and TSH.beta.. In populations exposed to vehicle only, 1-2% of mixed pituitary cells stored both ACTH and TSH. This unique cell cype also comprised 10% of a corticotrope population enriched by counterflow centrifugation. The percentage of ACTH-TSH cells in the mixed cell population was augmented to 4.8% after 1 h in AVP. It was not affected by exposure to either TRH or CRH (or both peptides). These studies demonstrate that AVP target cells include thyrotropes, corticotropes, and unique cells that store both ACTH and TSH.