Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin
Synthesis of fluorine substituted oligosaccharide analogues of monoglucosylated glycan chain, a proposed ligand of lectin-chaperone calreticulin and calnexin
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DOI：
10.1023/b:glyc.0000045109.60425.2e
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Takatani, M
中科院分区：
文献类型：
--
作者：
Ito, Y;Hagihara, S;Takatani, M
作为探索N-葡聚糖介导的内质网糖蛋白质量控制的一部分，以简明的方式合成了2-氟代衍生物Glcalpha1-->；3Man(F)1，Glcalpha1-->；3Man(F)alpha1-->；2Manan3。用等温滴定量热法研究了这些低聚糖与钙网蛋白的结合情况。结果表明，二糖1是一种较差的配体，而三-(2)和四糖(3)具有明显的亲和力。
As a part of a exploring the N-glycan-mediated glycoprotein quality control in endoplasmic reticulum, 2-fluorinated derivative Glcalpha1 --> 3Man(F) 1, Glcalpha1 --> 3Man(F) alpha1 --> 2Man2, and Glcalpha1 --> 3Man(F) alpha1 --> 2Manalpha1 --> 2Man 3 were synthesized in a concise manner. These oligosaccharides were subjected to binding studies with calreticulin by using isothermal titration calorimetry. It was revealed that disaccharide 1 was a poor ligand, while tri-( 2) and tetrasaccharide ( 3) had observable affinity.