Transcriptional analysis of antioxidant and immune defense genes in disk abalone (Haliotis discus discus) during thermal, low-salinity and hypoxic stress
Transcriptional analysis of antioxidant and immune defense genes in disk abalone (Haliotis discus discus) during thermal, low-salinity and hypoxic stress
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DOI：
10.1016/j.cbpb.2009.08.002
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发表时间：
2009-12-01
影响因子：
2.2
通讯作者：
Lee, Jehee
中科院分区：
文献类型：
--
作者：
De Zoysa, Mahanama;Whang, Ilson;Lee, Jehee
本研究描述了使用定量实时 PCR 对暴露于热、盐度和缺氧相关应激的圆盘鲍鱼鳃组织中的抗氧化和免疫防御基因进行转录分析。结果表明，锰超氧化物歧化酶（MnSOD）、铜锌超氧化物歧化酶（CuZnSOD）、过氧化氢酶（CAT）、硫氧还蛋白过氧化物酶（TPx）、硒依赖性谷胱甘肽过氧化物酶（SeGPx）和硫氧还蛋白-2（TRx-2）转录本在鲍鱼鳃中表达不同，并且它们共同作为鲍鱼的经典酶促抗氧化防御系统做出反应。表达谱的比较分析表明，相对于各自对照的水平，CAT、TPx 和 SeGPx 转录物在所有三种物理应激条件（热（28 摄氏度）、盐度（千分之 25）和缺氧）下均显着上调（p < 0.05）。相反，CuZnSOD 和 TRx-2 转录因热应激而下调。有趣的是，所有抗氧化剂转录本都表现出对盐度相关应激的显着上调。同时，缺氧导致 MnSOD、CAT、TPx: 和 SeGPx 上调，但不导致 CuZnSOD 和 TRx-2 转录本上调。最重要的结果之一是 SeGPx 的诱导量增加了 10 倍以上，这表明在物理压力条件下，SeGPx 是抗氧化酶中的潜在生物标志物基因。研究了物理应激对免疫功能基因转录反应的影响，即细胞因子信号传导抑制因子 2 (SOCS-2) 和粘病毒抗性 (Mx)。我们观察到盐度和缺氧都会增加 SOCS-2 和 Mx 转录本，而 28 摄氏度的热应激影响较小，产生混合的转录反应（上调和下调）。根据这些结果，我们假设鲍鱼共同利用抗氧化和免疫防御机制来克服物理压力。 (C) 2009 Elsevier Inc. 保留所有权利。
This study describes the transcriptional analysis of antioxidant and immune defense genes in gills tissue of disk abalone exposed to thermal, salinity and hypoxia-related stress, using quantitative real-time PCR. Results showed that manganese superoxide dismutase (MnSOD), copper zinc superoxide dismutase (CuZnSOD), catalase (CAT), thioredoxin peroxidase (TPx), selenium dependant glutathione peroxidase (SeGPx), and thioredoxin-2 (TRx-2) transcripts were expressed differently in gills, and that they respond collectively as a classical enzymatic antioxidant defense system in abalone. Comparative analysis of expression profiles indicated that CAT, TPx and SeGPx transcripts were significantly up-regulated (p < 0.05) by all three physical stress conditions - thermal (28 degrees C), salinity (25 parts per thousand) and hypoxia - relative to levels in respective controls. In contrast, CuZnSOD and TRx-2 transcription were down-regulated in response to thermal stress. Interestingly, all the antioxidant transcripts exhibited significant up-regulation in response to salinity-related stress. Meanwhile, hypoxia caused up-regulation of the MnSOD, CAT, TPx: and SeGPx, but not the CuZnSOD and TRx-2 transcripts. One of the most significant outcomes was the more than 10-fold induction of SeGPx, suggesting that SeGPx is a potential biomarker gene among antioxidant enzymes, under conditions of physical stress. The effects of physical stress on the transcriptional responses of immune functional genes namely suppressor of cytokine signaling-2 (SOCS-2) and myxovirus resistance (Mx) were investigated. We observed that salinity and hypoxia increase both the SOCS-2 and Mx transcripts, while thermal stress at 28 degrees C exerts less of an effect, producing mixed transcriptional responses (both up and down regulation). Based upon these results, we postulate that abalones utilize antioxidant and immune defense mechanisms together to overcome physical stresses. (C) 2009 Elsevier Inc. All rights reserved.