Ventral otic cell lines as developmental models of auditory epithelial and neural precursors
Ventral otic cell lines as developmental models of auditory epithelial and neural precursors
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DOI：
10.1002/dvdy.20187
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
Holley, MC
中科院分区：
文献类型：
--
作者：
Lawoko-Kerali, G;Milo, M;Holley, MC
从胚胎第10.5天的Immortomouse腹侧耳囊中建立了连续永生细胞系，并选择其代表听觉感觉神经和上皮细胞的前体细胞。选择是基于解剖，组织特异性标记，和转录因子GATA 3的表达。两个细胞系表达GATA 3，但在分化条件下具有本质上不同的遗传程序。US/VOT-E36代表具有分化为感觉和非感觉上皮细胞的潜能的上皮祖细胞。US/VOT-N33代表迁移的成神经细胞。在体外分化条件下，细胞系表达非常不同的基因表达谱。几种细胞和组织特异性标志物的表达，包括转录因子Pax 2、GATA 3和NeuroD，在细胞系之间的差异与在体内它们的对应物之间观察到的模式一致。我们认为，这些和其他条件永生细胞系可用于研究瞬态事件的发展对不同背景的细胞能力。(C)2004 Wiley-Liss，Inc.
Conditionally immortal cell lines were established from the ventral otocyst of the Immortomouse at embryonic day 10.5 and selected to represent precursors of auditory sensory neural and epithelial cells. Selection was based upon dissection, tissue-specific markers, and expression of the transcription factor GATA3. Two cell lines expressed GATA3 but possessed intrinsically different genetic programs under differentiating conditions. US/VOT-E36 represented epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells. US/VOT-N33 represented migrating neuroblasts. Under differentiating conditions in vitro the cell lines expressed very different gene expression profiles. Expression Of Several cell- and tissue-specific markers, including the transcription factors Pax2, GATA3, and NeuroD, differed between the cell lines in a pattern consistent with that observed between their counterparts in vivo. We suggest that these and other conditionally immortal cell lines can be used to study transient events in development against different backgrounds of cell competence. (C) 2004 Wiley-Liss, Inc.