Transfection by electroporation.
Transfection by electroporation.
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DOI：
10.1002/0471142727.mb0903s62
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发表时间：
2003-05-01
影响因子：
--
通讯作者：
Potter, Huntington
中科院分区：
文献类型：
--
作者：
Potter, Huntington
电穿孔-使用高压电击将DNA引入细胞-可用于大多数细胞类型，产生高频率的稳定转化和瞬时基因表达，并且因为它需要更少的步骤，所以比其他技术更容易。在本单元中，描述了哺乳动物细胞的电穿孔，并概述了植物原生质体制备和转染的修改。
Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. In this unit, the describes the electroporation of mammalian cells and an outlines modifications for preparation and transfection of plant protoplasts.