Microbes on the human vaginal epithelium
Microbes on the human vaginal epithelium
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DOI：
10.1073/pnas.0503236102
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发表时间：
2005-05-31
影响因子：
11.1
通讯作者：
Davis, RW
中科院分区：
文献类型：
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作者：
Hyman, RW;Fukushima, M;Davis, RW
仅使用基于基因的程序，对16S核糖体RNA基因进行PCR扩增，并对扩增产物进行深度测序，对20例健康女性阴道上皮上的微生物进行了鉴定和定量。这20个健康阴道上皮细胞的乳酸杆菌含量变化很大，从0%到100%不等。对于四名受试者，乳杆菌（实际上）是唯一检测到的细菌。然而，该乳酸菌远非无性系，而是种和菌株的混合物。8名受试者呈现乳酸杆菌和其他微生物的复杂混合物。其余8名受试者没有乳酸菌。相反，双歧杆菌、加德纳菌、普雷沃氏菌、假单胞菌或链球菌占主导地位。
Using solely a gene-based procedure, PCR amplification of the 16S ribosomal RNA gene coupled with very deep sequencing of the amplified products, the microbes on 20 human vaginal epithelia of healthy women have been identified and quantitated. The Lactobacillus content on these 20 healthy vaginal epithelia was highly variable, ranging from 0% to 100%. For four subjects, Lactobacillus was (virtually) the only bacterium detected. However, that Lactobacillus was far from clonal and was a mixture of species and strains. Eight subjects presented complex mixtures of Lactobacillus and other microbes. The remaining eight subjects had no Lactobacillus. Instead, Bifidobacterium, Gardnerella, Prevotella, Pseudomonas, or Streptococcus predominated.