Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by Cyclin E
Expression of the cyclin-dependent kinase inhibitor Dacapo is regulated by Cyclin E
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DOI：
10.1016/s0925-4773(00)00435-4
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Hariharan, IK
中科院分区：
文献类型：
--
作者：
de Nooij, JC;Graber, KH;Hariharan, IK
细胞周期蛋白依赖性激酶抑制剂 (CKI) 的 Cip/Kip 家族参与介导终末分化之前的细胞周期停滞。在许多情况下，CKI 表达增加立即发生在有丝分裂停滞之前。然而，在即将停止分裂的细胞中激活 CKI 表达的机制仍然难以捉摸。在这里，我们通过研究 dacapo（一种 Cip/Kip CKI）在果蝇中的表达模式来解决这个问题。我们发现 dacapo RNA 和蛋白质的积累需要 Cyclin E，并且 Cyclin E 表达的增加可以诱导 dacapo 表达。我们还表明，Cyclin E 和 Dacapo 蛋白的振荡在卵巢内周期过程中紧密耦合。我们的结果证明了 Cyclin E/Cdk 活性诱导 Dacapo 表达的机制，但仅在允许 dacapo 表达的某些窗口内。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
The Cip/Kip family of cyclin-dependent kinase inhibitors (CKIs) has been implicated in mediating cell cycle arrest prior to terminal differentiation. In many instances, increased expression of CKIs immediately precedes mitotic arrest. However, the mechanism that activates CKI expression in cells that are about to stop dividing has remained elusive. Here we have addressed this issue by investigating the expression pattern of dacapo, a Cip/Kip CKI in Drosophila. We show that the accumulation of dacapo RNA and protein requires Cyclin E and that increased expression of Cyclin E can induce dacapo expression. We also show that the oscillation of the Cyclin E and Dacapo proteins are tightly coupled during ovarian endocycles. Our results argue for a mechanism where Cyclin E/Cdk activity induces Dacapo expression but only within certain windows that are permissive for dacapo expression. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.