Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II.
Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II.
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新型人类巨噬细胞系 K1m 的表型和功能表征，展示了通过 HLA II 类的免疫吞噬活性和信号传导。
DOI：
10.1046/j.1365-2567.1997.00185.x
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发表时间：
1997
期刊：
影响因子：
6.4
通讯作者：
Yu,J
中科院分区：
文献类型：
--
作者：
Dialynas,DP;Lee,MJ;Shao,LE;Tan,PC;Yu,J
人巨噬细胞系，命名为K1 m，已建立从外周血。K1 m表达许多谱系特异性标志物以及广泛的细胞间粘附分子。特别是，K1 m表达高水平的人类白细胞抗原（HLA）I类和II类。在对HLA II类（HLA-DR）连接的反应中，而不是对HLA I类连接的反应中，K1 m形成更紧密的同型聚集体，并形成显著的“星状”培养表型。K1 m还表达免疫球蛋白G（IgG）的Fc受体（CD 64、CD 32和CD 16），并且可以在存在肿瘤特异性单克隆抗体（mAb）的情况下吞噬聚苯乙烯乳胶珠以及神经母细胞瘤细胞。因此，K1 m细胞系应证明可用于研究通过巨噬细胞HLA II类和免疫吞噬作用的信号传导。
A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage‐specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA‐DR), but not in response to ligation of HLA class I, K1m forms tighter homotypic aggregates and develops a striking ‘stellate’ culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour‐specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.