Modeling electroporation in a single cell. I. Effects of field strength and rest potential
Modeling electroporation in a single cell. I. Effects of field strength and rest potential
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DOI：
10.1016/s0006-3495(99)76973-0
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Krassowska, W
中科院分区：
文献类型：
--
作者：
DeBruin, KA;Krassowska, W
本研究建立了一个外电场电穿孔单细胞模型，并利用该模型研究了冲击强度和静息电位对细胞跨膜电位V-m和孔密度N的影响。与电容-电容理论预测的诱导电位相比，电穿孔模型预测整个细胞中的V-m的幅度较小。V-m和N都是关于赤道对称的，在单元的两极具有相同的值。更大的冲击不会增加V-m的最大幅度，因为形成更多的孔以分流穿过膜的过量刺激电流。此外，静止电位的值不影响细胞周围的V-m，因为电穿孔电流比支持静止电位的离子电流大几个数量级。一旦场被移除，冲击诱导的V-m在2 μ s内放电，但是孔在膜中持续几秒钟。完全重新密封至震前状态需要大约20 s。这些结果同意定性和定量与Kinosita和同事报告的未受精的海胆卵暴露于大电场的实验数据。
This study develops a model for a single cell electroporated by an external electric field and uses it to investigate the effects of shock strength and rest potential on the transmembrane potential V-m and pore density N around the cell. As compared to the induced potential predicted by resistive-capacitive theory, the model of electroporation predicts a smaller magnitude of V-m throughout the cell. Both V-m and N are symmetric about the equator with the same Value at both poles of the cell. Larger shocks do not increase the maximum magnitude of V-m because more pores form to shunt the excess stimulus current across the membrane. In addition, the value of the rest potential does not affect V-m around the cell because the electroporation current is several orders of magnitude larger than the ionic current that supports the rest potential. Once the field is removed, the shock-induced V-m discharges within 2 mu s, but the pores persist in the membrane for several seconds. Complete resealing to preshock conditions requires approximately 20 s. These results agree qualitatively and quantitatively with the experimental data reported by Kinosita and coworkers for unfertilized sea urchin eggs exposed to large electric fields.