Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging.
Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging.
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体内多模式视网膜成像的光学相干光学显微镜。
DOI：
10.1364/ol.40.001370
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发表时间：
2015-04-01
期刊：
影响因子：
3.6
通讯作者：
Jiao S
中科院分区：
文献类型：
--
作者：
Liu X;Liu T;Wen R;Li Y;Puliafito CA;Zhang HF;Jiao S
研制了一种光相干光声显微镜（OC-PAM）系统，该系统利用一个脉冲宽带光源同时实现了光学相干层析成像（OCT）和光声显微镜（PAM）的检测。该系统的中心波长为800 nm，带宽为30 nm，适用于视网膜成像。OCT和PAM图像由同一组光子生成，在横向方向上固有地配准。为了测试系统在多模态眼科成像上的能力，我们对有色大鼠的视网膜进行成像。OCT图像显示了与传统OCT质量相似的视网膜结构，而PAM图像显示了视网膜中吸收体的分布。由于血红蛋白的吸收在约800 nm处相对较弱，因此NIR PAM信号主要由眼睛后段中的黑色素产生，从而提供视网膜的黑色素特异性成像。
We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.