Preparation and characterization novel polymer-coated magnetic nanoparticles as carriers for doxorubicin
Preparation and characterization novel polymer-coated magnetic nanoparticles as carriers for doxorubicin
复制标题
DOI：
10.1016/j.colsurfb.2011.06.003
复制
发表时间：
2011-11-01
影响因子：
5.8
通讯作者：
Shi, Donglu
中科院分区：
文献类型：
--
作者：
Li, Fang;Sun, Jing;Shi, Donglu
采用水包油包水（W/O/W）乳化法制备了聚（丙交酯-乙交酯）磁性纳米粒（PLGA MNP）作为阿霉素的载体（PLGA DOX MNP）。用透射电镜（TEM）和振动取样磁强计（VSM）对PLGA-DOX MNP的特性进行了测量。结果表明，所合成的纳米粒子为球形，平均粒径为100 ± 20 nm，聚集度低，磁响应性好。同时，纳米粒的药物含量和包封率可以通过改变PLGA和DOX颗粒的进料重量比来实现。这些PLGA-DOX MNP还显示在缓冲溶液中在37 ° C下持续释放DOX。MTT法检测载药纳米粒对体外细胞毒性的影响，Annexin V-FITC凋亡检测试剂盒检测细胞凋亡。结果表明，PLGA-DOX MNP保留了显著的抗肿瘤活性。因此，PLGA-DOX MNP可能被认为是一种有前途的肿瘤化疗药物递送系统。(C)2011 Elsevier B. V.保留所有权利。
The poly(lactide-co-glycolide)-coated magnetic nanoparticles (PLGA MNPs) were prepared as carriers of doxorubicin (PLGA-DOX MNPs) through water-in-oil-in-water (W/O/W) emulsification method. The characteristics of PLGA-DOX MNPs were measured by using transmission electron microscopy (TEM) and vibrating-sampling magnetometry (VSM). It was found that the synthesized nanoparticles were spherical in shape with an average size of 100 +/- 20 nm, low aggregation and good magnetic responsivity. Meanwhile, the drug content and encapsulation efficiency of nanoparticles can be achieved by varying the feed weight ratios of PLGA and DOX particles. These PLGA-DOX MNPs also demonstrated sustained release of DOX at 37 degrees C in buffer solution. Besides, influence of drug-loaded nanoparticles on in vitro cytotoxicity was determined by MTT assay, while cellular apoptosis was detected by Annexin V-FITC apoptosis detection kit. The results showed that PLGA-DOX MNPs retained significant antitumor activities. Therefore, PLGA-DOX MNPs might be considered a promising drug delivery system for cancer chemotherapy. (C) 2011 Elsevier B.V. All rights reserved.