CHARACTERIZATION OF GENE-EXPRESSION IN CLINICAL LUNG-CANCER MATERIALS BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS
CHARACTERIZATION OF GENE-EXPRESSION IN CLINICAL LUNG-CANCER MATERIALS BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1002/elps.1150150157
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发表时间：
1994-03-01
期刊：
影响因子：
2.9
通讯作者：
AUER, G
中科院分区：
文献类型：
--
作者：
OKUZAWA, K;FRANZEN, B;AUER, G
对14例不同组织病理类型的人肺肿瘤进行了双向凝胶电泳（2-DE）。样品制备2-DE使用非酶样品制备（NESP）技术最近建立在我们的实验室。在不同组织病理类型的肿瘤之间观察到一些多肽的表达的变化。为此，在小细胞肺癌（SCLC）中观察到β-微管蛋白、热休克蛋白73和90、核纤层蛋白B和增殖细胞核抗原（PCNA）的高表达。一个未知的身份（35 kDa，pI 5.5）的多肽显着过表达的原发性肺腺癌相比，小细胞肺癌，鳞状细胞肺癌，转移性肺腺癌从结肠和直肠，和正常组织。给出了该多肽的氨基酸组成。总之，NESP技术和2-DE技术相结合是确定肿瘤特异性标志物的有效方法。
Fourteen human lung tumors of various histopathological types were subjected to two-dimensional gel electrophoresis (2-DE). Samples were prepared for 2-DE using a nonenzymatic sample preparation (NESP) technique recently established in our laboratory. Variations in the expression of some polypeptides were observed between tumors of different histopathological types. To this end, high expression of beta-tubulin, heat shock proteins 73 and 90, lamin B, and proliferating cell nuclear antigen (PCNA) were observed in small cell lung carcinomas (SCLC). One polypeptide of unknown identity (35 kDa, pI 5.5) was significantly overexpressed in primary lung adenocarcinomas compared with SCLC, squamous cell lung carcinomas, metastatic lung adenocarcinomas from colon and rectum, and normal tissue. The amino acid composition of this polypeptide is presented. In summary, combining the NESP technique and 2-DE is an effective approach to define tumor-specific markers.