Quantum measurement and orientation tracking of fluorescent nanodiamonds inside living cells
Quantum measurement and orientation tracking of fluorescent nanodiamonds inside living cells
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DOI：
10.1038/nnano.2011.64
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发表时间：
2011-06-01
影响因子：
38.3
通讯作者：
Hollenberg, L. C. L.
中科院分区：
文献类型：
--
作者：
McGuinness, L. P.;Yan, Y.;Hollenberg, L. C. L.
荧光颗粒通常用于探测生物过程（1）。荧光颗粒内单自旋的量子特性已经在纳米级磁力测量领域进行了探索（2-8），但尚未在生物环境中进行。在这里，我们展示了活体人类HeLa细胞内单个荧光纳米金刚石氮空位中心的光学检测磁共振，并以纳米级精度测量它们的位置，方向，自旋水平和自旋相干时间。量子相干性通过拉比和自旋回波序列在长时间（>10小时）内测量，并在89 ms的采集时间内以1度的有效角精度跟踪方向。量子自旋水平作为指纹，允许同时识别和跟踪具有相同荧光的各个中心。此外，监测响应于局部环境变化的去相干速率可以提供关于细胞内过程的新信息。这里报道的实验证明了受控单自旋探针在生物系统中用于纳米磁测量的可行性，为生命科学中基于量子的成像开辟了许多新的可能性。
Fluorescent particles are routinely used to probe biological processes(1). The quantum properties of single spins within fluorescent particles have been explored in the field of nanoscale magnetometry(2-8), but not yet in biological environments. Here, we demonstrate optically detected magnetic resonance of individual fluorescent nanodiamond nitrogen-vacancy centres inside living human HeLa cells, and measure their location, orientation, spin levels and spin coherence times with nanoscale precision. Quantum coherence was measured through Rabi and spin-echo sequences over long (>10 h) periods, and orientation was tracked with effective 1 degrees angular precision over acquisition times of 89 ms. The quantum spin levels served as fingerprints, allowing individual centres with identical fluorescence to be identified and tracked simultaneously. Furthermore, monitoring decoherence rates in response to changes in the local environment may provide new information about intracellular processes. The experiments reported here demonstrate the viability of controlled single spin probes for nanomagnetometry in biological systems, opening up a host of new possibilities for quantum-based imaging in the life sciences.