Global Distribution and Evolution of Panton-Valentine Leukocidin-Positive Methicillin-Susceptible Staphylococcus aureus, 1981-2007
Global Distribution and Evolution of Panton-Valentine Leukocidin-Positive Methicillin-Susceptible Staphylococcus aureus, 1981-2007
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DOI：
10.1086/652008
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发表时间：
2010-05-15
影响因子：
6.4
通讯作者：
Tristan, Anne
中科院分区：
文献类型：
--
作者：
Rasigade, Jean-Philippe;Laurent, Frederic;Tristan, Anne
背景资料。潘顿-瓦伦丁杀白素(PVL)阳性的甲氧西林敏感金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(分别为MSSA和MRSA)都与严重感染有关，如坏死性肺炎。PVL阳性社区获得性耐甲氧西林金黄色葡萄球菌(CA)的流行病学特征已被广泛研究，但关于PVL阳性MSSA的相应数据很少。本研究的目的是调查PVL阳性MSSA的全球种群结构，并将其与已报道的CA-MRSA进行比较，从而探讨这些病原体之间的系统发育关系。我们测定了1981-2007年间在全球19个国家和地区收集的211株PVL阳性MSSA临床分离株的agr类型、多位点序列类型和毒素基因图谱。PVL阳性MSSA的优势谱系为agr3/ST30、agr4/ST121、agr3/st1、agr2/st5和agr3/ST80。据报道，除agr4/ST121外，这些谱系也在CA-MRSA中流行。在过去的20年里，与CA-MRSA有遗传关系的PVL阳性的MSSA谱系逐渐取代了其他谱系(特别是agr4/ST121)。在给定的序列类型内，PVL阳性的MSSA株与PVL阳性的CA-MRSA株的毒素基因含量非常相似。PVL阳性的MSSA和CA-MRSA的分子流行病学特征是动态相关的，前者似乎构成了后者的储备库。
Background. Panton-Valentine leukocidin (PVL)-positive methicillin-susceptible Staphylococcus aureus and methicillin-resistant S. aureus (MSSA and MRSA, respectively) are both associated with severe infections, such as necrotizing pneumonia. The epidemiological profile of PVL-positive community-acquired (CA) MRSA has been extensively studied, but few corresponding data on PVL-positive MSSA are available.Objectives. The objectives of the study were to investigate the global population structure of PVL-positive MSSA, to compare it with that reported for CA-MRSA, and thus to examine the phylogenetic relationship between these pathogens.Methods. We determined the agr types, multilocus sequence types, and toxin gene profiles of 211 PVL-positive MSSA clinical isolates collected in 19 countries throughout the world between 1981 and 2007.Results. The predominant lineages of PVL-positive MSSA were agr3/ST30, agr4/ST121, agr3/ST1, agr2/ST5, and agr3/ST80. Except for agr4/ST121, these lineages are also reported to be prevalent among CA-MRSA. PVL-positive MSSA lineages that are genetically related to CA-MRSA have gradually replaced other lineages (especially agr4/ST121) over the past 2 decades. Within a given sequence type, the toxin gene content of PVL-positive MSSA strains was very similar to that of PVL-positive CA-MRSA.Conclusions. The molecular epidemiological profiles of PVL-positive MSSA and CA-MRSA are dynamically interrelated, with the former appearing to constitute a reservoir for the latter.