Prevention of Vocal Fold Scarring by Local Application of Basic Fibroblast Growth Factor in a Rat Vocal Fold Injury Model
Prevention of Vocal Fold Scarring by Local Application of Basic Fibroblast Growth Factor in a Rat Vocal Fold Injury Model
复制标题
DOI：
10.1002/lary.26138
复制
发表时间：
2017-02-01
期刊：
影响因子：
2.6
通讯作者：
Hirano, Shigeru
中科院分区：
文献类型：
--
作者：
Suzuki, Ryo;Kawai, Yoshitaka;Hirano, Shigeru
目的/假设：声带瘢痕是一种难治性的瘢痕，可导致严重的声音嘶哑。声带瘢痕的最佳治疗方法尚未确定；因此，预防疤痕是很重要的。本研究的目的是阐明碱性成纤维细胞生长因子（bFGF）预防术后声带瘢痕形成的有效性。研究设计：前瞻性对照动物实验。方法：局部应用10 μ L bFGF溶液损伤sd - dawley大鼠单侧或双侧声带。术后2个月切除喉部进行组织学和免疫组织化学检查，术后1周进行实时定量聚合酶链反应（qRT-PCR）分析。结果：组织学检查显示，与假药组相比，100 ng/10 μ L bfgf处理组透明质酸明显增加，致密胶原沉积明显减少。免疫组化检查显示，与假药组相比，100 ng/10 μ L bfgf处理组III型胶原沉积明显减少。qRT-PCR结果显示，与假药组相比，bfgf治疗组的透明质酸合成酶2 （Has2）、Has3和肝细胞生长因子表达上调。结论：损伤时局部应用bFGF具有预防声带瘢痕形成的作用。微创手术时可预防性注射bFGF，避免术后瘢痕形成。
Objectives/Hypothesis: Vocal fold scarring, which causes severe hoarseness, is intractable. The optimal treatment for vocal fold scarring has not been established; therefore, prevention of scarring is important. The aim of this study was to clarify the effectiveness of basic fibroblast growth factor ( bFGF) for prevention of postsurgical vocal fold scarring.Study Design: Prospective animal experiments with controls.Method: The vocal folds of Sprague-Dawley rats were injured unilaterally or bilaterally after local application of a 10 mu L solution of bFGF. Larynges ware harvested for histological and immunohistochemical examination 2 months postoperation and for quantitative real-time polymerase chain reaction ( qRT-PCR) analysis 1 week postoperation.Results: Histological examination showed significantly increased hyaluronic acid and decreased deposition of dense collagen in the bFGF-treated group at 100 ng/10 mu L compared with the sham-treated group. Immunohistochemical examination showed significantly decreased collagen type III deposition in the bFGF-treated group at 100 ng/10 mu L compared with the sham-treated group. qRT-PCR revealed that hyaluronan synthase 2 ( Has2), Has3, and hepatocyte growth factor were upregulated in bFGF-treated groups compared with sham-treated group.Conclusion: The current results suggest that local application of bFGF at the time of injury has the potential to prevent vocal fold scarring. Preventive injection of bFGF could be applied at the time of phonomicrosurgery to avoid postoperative scar formation.